BMRB Entry 25610

Name: NMR solution structure of the pheromone Ep-1 from Euplotes petzi
Published: 2016-04-25

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PDB ID: 2n2s
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR25610
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR solution structure of the pheromone Ep-1 from Euplotes petzi

Deposition date:

2015-05-14

Original release date:

2016-04-25

Authors:

Pedrini, Bill; Vallesi, Adriana; Alimenti, Claudio; Luporini, Pierangelo

Citation:

Citation: Pedrini, Bill; Suter-Stahel, Thea; Vallesi, Adriana; Alimenti, Claudio; Luporini, Pierangelo. "Molecular Structures and Coding Genes of the Water-Borne Protein Pheromones of Euplotes petzi, an Early Diverging Polar Species of Euplotes" J. Eukaryot. Microbiol. 64, 164-172 (2017).
PubMed: 27455370

Assembly members:

Assembly members:
Ep-1, polymer, 32 residues, 3180.605 Da.

Natural source:

Natural source: Common Name: ciliates Taxonomy ID: 1156992 Superkingdom: Eukaryota Kingdom: not available Genus/species: Euplotes petzi

Experimental source:

Experimental source: Production method: purified from the natural source Host organism: Euplotes petzi

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ep-1: SCGSECAPEPDCWGCCLVQC APSICAGWCGGS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	163

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity	1

Entities:

Entity 1, entity 32 residues - 3180.605 Da.

1

SER

CYS

GLY

SER

GLU

CYS

ALA

PRO

GLU

PRO

2

ASP

CYS

TRP

GLY

CYS

LEU

VAL

GLN

CYS

3

ALA

PRO

SER

ILE

CYS

ALA

GLY

TRP

CYS

GLY

4

GLY

SER

Samples:

sample_1: Ep-1 0.25 ± 0.05 mM; H2O 95%; D2O, [U-2H], 5%

sample_conditions_1: ionic strength: 0.06 M; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D DQF-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger A. T. et.al., Keller and Wuthrich - chemical shift assignment, refinement

ATNOS, Guentert, P, Herrmann, T, Wuethrich, K - peak picking

CANDID, Guentert, P, Herrmann, T, Wuethrich, K - peak assignment, upper distance constraint determination

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 600 MHz