BMRB Entry 25586

Title:
Backbone resonance assignments of the wt NS4A N-terminal domain of DENV in aqueous solution
Deposition date:
2015-04-28
Original release date:
2015-07-24
Authors:
Hung, Yu-Fu; Schwarten, Melanie; Hoffmann, Silke; Willbold, Dieter; Sklan, Ella; Koenig, Bernd
Citation:

Citation: Hung, Yu-Fu; Schwarten, Melanie; Hoffmann, Silke; Sklan, Ella; Willbold, Dieter; Koenig, Bernd. "Amino Terminal Region of Dengue Virus NS4A Cytosolic Domain Binds to Highly Curved Liposomes."  Viruses 7, 4119-4130 (2015).
PubMed: 26197333

Assembly members:

Assembly members:
Molecule_1, polymer, 48 residues, 5226.9633 Da.

Natural source:

Natural source:   Common Name: DENV Type 2   Taxonomy ID: 56636   Superkingdom: Archaea   Kingdom: not available   Genus/species: Aeropyrum pernix

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Molecule_1: SLTLNLITEMGRLPTFMTQK ARDALDNLAVLHTAEAGGRA YNHALSEL

Data sets:
Data typeCount
13C chemical shifts92
15N chemical shifts41
1H chemical shifts41

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1wt NS4A N-terminal domain of DENV1

Entities:

Entity 1, wt NS4A N-terminal domain of DENV 48 residues - 5226.9633 Da.

1   SERLEUTHRLEUASNLEUILETHRGLUMET
2   GLYARGLEUPROTHRPHEMETTHRGLNLYS
3   ALAARGASPALALEUASPASNLEUALAVAL
4   LEUHISTHRALAGLUALAGLYGLYARGALA
5   TYRASNHISALALEUSERGLULEU

Samples:

NMR_sample: wtNS4A, [U-13C; U-15N], 200 uM; Sodium Phosphate 50 mM; Sodium Azide 0.03%; Sodium Chloride 150 mM

NMR_sample_ref: wtNS4A, [U-13C; U-15N], 200 uM; Sodium Phosphate 50 mM; DSS 10 mM; Sodium Azide 0.03%; Sodium Chloride 150 mM

Set_1: ionic strength: 200 mM; pH: 6.8; pressure: 1.000 atm; temperature: 303.0 K

Set_2: ionic strength: 200 mM; pH: 6.8; pressure: 1.000 atm; temperature: 303.0 K

Experiments:

NameSampleSample stateSample conditions
3D HNCANMR_sampleisotropicSet_1
2D 1H-15N HSQC/HMQCNMR_sampleisotropicSet_1
3D HNCONMR_sampleisotropicSet_1
HNcaCO (H[N[ca[CO]]])NMR_sampleisotropicSet_1
2D 1H-13C HSQC/HMQCNMR_sample_refisotropicSet_2
2D 1H-15N HSQC/HMQCNMR_sample_refisotropicSet_2

Software:

CcpNmr_Analysis v2.4, CCPN - chemical shift assignment

NMR spectrometers:

  • Varian VNMRS 900 MHz
  • Bruker Bruker Avance III HD 700 MHz

Related Database Links:

BMRB 25179
DBJ BAD42415 BAD42416 BAD42417 BAH86603 BAH86604
EMBL CAD31751 CAI92123 CAR65133 CAR65134 CAR65135
GB AAA42941 AAA42942 AAA42962 AAA73185 AAA73186
REF NP_056776 NP_739588
SP P07564 P12823 P14337 P14340 P29990
AlphaFold P07564 P12823 P14337 P14340 P29990

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks