BMRB Entry 25572
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25572
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Citation: Singh, Himanshu. "Dad2 C terminal" Biochemistry ., .-..
Assembly members:
Dad2_C_terminal, polymer, 139 residues, Formula weight is not available
Natural source: Common Name: Nematodes Taxonomy ID: 6239 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Caenorhabditis elegans
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28
Entity Sequences (FASTA):
Dad2_C_terminal: MASKTNTAIYQKIAEKRANL
ERFREFKELTDDLVLQLESI
GDKLETMNGGTASVALILAN
WKSVVQSISLASLALMKESN
DNNKEAFPEPLVRVRVGQSN
EENQDEEEADEEEGVRDSEE
VEESTEKLAAALEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 115 |
| 15N chemical shifts | 49 |
| 1H chemical shifts | 50 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 139 residues - Formula weight is not available
| 1 | MET | ALA | SER | LYS | THR | ASN | THR | ALA | ILE | TYR | ||||
| 2 | GLN | LYS | ILE | ALA | GLU | LYS | ARG | ALA | ASN | LEU | ||||
| 3 | GLU | ARG | PHE | ARG | GLU | PHE | LYS | GLU | LEU | THR | ||||
| 4 | ASP | ASP | LEU | VAL | LEU | GLN | LEU | GLU | SER | ILE | ||||
| 5 | GLY | ASP | LYS | LEU | GLU | THR | MET | ASN | GLY | GLY | ||||
| 6 | THR | ALA | SER | VAL | ALA | LEU | ILE | LEU | ALA | ASN | ||||
| 7 | TRP | LYS | SER | VAL | VAL | GLN | SER | ILE | SER | LEU | ||||
| 8 | ALA | SER | LEU | ALA | LEU | MET | LYS | GLU | SER | ASN | ||||
| 9 | ASP | ASN | ASN | LYS | GLU | ALA | PHE | PRO | GLU | PRO | ||||
| 10 | LEU | VAL | ARG | VAL | ARG | VAL | GLY | GLN | SER | ASN | ||||
| 11 | GLU | GLU | ASN | GLN | ASP | GLU | GLU | GLU | ALA | ASP | ||||
| 12 | GLU | GLU | GLU | GLY | VAL | ARG | ASP | SER | GLU | GLU | ||||
| 13 | VAL | GLU | GLU | SER | THR | GLU | LYS | LEU | ALA | ALA | ||||
| 14 | ALA | LEU | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: entity, [U-100% 15N], .4 mM; entity, [U-100% 13C], .2 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.1 M; pH: 2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
cara, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| EMBL | CAX43751 |
| GB | EAK95870 EEQ45932 KGQ89068 KGQ96391 KGR00135 |
| REF | XP_002418450 XP_714910 |
| SP | Q59ZG8 |
| AlphaFold | Q59ZG8 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks