BMRB Entry 25560

Name: Solution structure of the GBII-beta MRH domain W409A point mutant
Published: 2015-12-07

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PDB ID: 2n1h
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25560
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the GBII-beta MRH domain W409A point mutant

Deposition date:

2015-04-01

Original release date:

2015-12-07

Authors:

Olson, Linda; Peterson, Francis; Damhs, Nancy

Citation:

Citation: Olson, Linda; Orsi, Ramiro; Peterson, Francis; Parodi, Armando; Kim, Jung-Ja; D'Alessio, Cecilia; Damhs, Nancy. "Crystal structure and functional analyses of glucosdidase II's lectin domain: Insgihts into oligomannose recognition" .

Assembly members:

Assembly members:
entity, polymer, 94 residues, 10428.918 Da.

Natural source:

Natural source: Common Name: fission yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: YRAIKGMETKREIGGYTYKV VFYENVFQDSILLGNFASQE GNVLKYENGQSCANGPHRSA IVTVECGVENEIVSVLEAQK CEYLIKMKSPAACS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	356
15N chemical shifts	96
1H chemical shifts	619

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity	1

Entities:

Entity 1, entity 94 residues - 10428.918 Da.

1

TYR

ARG

ALA

ILE

LYS

GLY

MET

GLU

THR

LYS

2

ARG

GLU

ILE

GLY

TYR

THR

TYR

LYS

VAL

3

VAL

PHE

TYR

GLU

ASN

VAL

PHE

GLN

ASP

SER

4

ILE

LEU

GLY

ASN

PHE

ALA

SER

GLN

GLU

5

GLY

ASN

VAL

LEU

LYS

TYR

GLU

ASN

GLY

GLN

6

SER

CYS

ALA

ASN

GLY

PRO

HIS

ARG

SER

ALA

7

ILE

VAL

THR

VAL

GLU

CYS

GLY

VAL

GLU

ASN

8

GLU

ILE

VAL

SER

VAL

LEU

GLU

ALA

GLN

LYS

9

CYS

GLU

TYR

LEU

ILE

LYS

MET

LYS

SER

PRO

10

ALA

CYS

SER

Samples:

sample_1: MRH GIIb, [U-99% 13C; U-99% 15N], 0.7 mM; imidazole, [U-99% 2H], 10 mM; sodium chloride 150 mM; sodium azide 0.02%; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.150 M; pH: 7.1; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

Software:

CYANA, Guentert P. - refinement

NMR spectrometers:

Bruker DRX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks