BMRB Entry 25479

Name: Role of a non-canonical surface of Rad6 in ubiquitin conjugating activity
Published: 2016-06-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25479

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Role of a non-canonical surface of Rad6 in ubiquitin conjugating activity

Deposition date:

2015-02-09

Original release date:

2016-06-30

Authors:

Kumar, Pankaj; Magala, Pearl; Gieger, Kathryn; Majumdar, Ananya; Tolman, Joel; Wolberger, Cynthia

Citation:

Citation: Kumar, Pankaj; Magala, Pearl; Gieger, Kathryn; Majumdar, Ananya; Tolman, Joel; Wolberger, Cynthia. "Role of a non-canonical surface of Rad6 in ubiquitin conjugating activity" Nucleic Acids Res. 43, 9039-9050 (2015).
PubMed: 26286193

Assembly members:

Assembly members:
Rad6, polymer, 169 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMALc2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rad6: GSMSTPARRRLMRDFKRMKE DAPPGVSASPLPDNVMVWNA MIIGPADTPYEDGTFRLLLE FDEEYPNKPPHVKFLSEMFH PNVYANGEICLDILQNRWTP TYDVASILTSIQSLFNDPNP ASPANVEAATLFKDHKSQYV KRVKETVEKSWEDDMDDMDD DDDDDDDDD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	76
1H chemical shifts	76

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Rad6	1

Entities:

Entity 1, Rad6 169 residues - Formula weight is not available

1

GLY

SER

MET

SER

THR

PRO

ALA

ARG

2

LEU

MET

ARG

ASP

PHE

LYS

ARG

MET

LYS

GLU

3

ASP

ALA

PRO

GLY

VAL

SER

ALA

SER

PRO

4

LEU

PRO

ASP

ASN

VAL

MET

VAL

TRP

ASN

ALA

5

MET

ILE

GLY

PRO

ALA

ASP

THR

PRO

TYR

6

GLU

ASP

GLY

THR

PHE

ARG

LEU

GLU

7

PHE

ASP

GLU

TYR

PRO

ASN

LYS

PRO

8

HIS

VAL

LYS

PHE

LEU

SER

GLU

MET

PHE

HIS

9

PRO

ASN

VAL

TYR

ALA

ASN

GLY

GLU

ILE

CYS

10

LEU

ASP

ILE

LEU

GLN

ASN

ARG

TRP

THR

PRO

11

THR

TYR

ASP

VAL

ALA

SER

ILE

LEU

THR

SER

12

ILE

GLN

SER

LEU

PHE

ASN

ASP

PRO

ASN

PRO

13

ALA

SER

PRO

ALA

ASN

VAL

GLU

ALA

THR

14

LEU

PHE

LYS

ASP

HIS

LYS

SER

GLN

TYR

VAL

15

LYS

ARG

VAL

LYS

GLU

THR

VAL

GLU

LYS

SER

16

TRP

GLU

ASP

MET

ASP

MET

ASP

17

ASP

Samples:

sample_1: Rad6, [U-100% 15N], 0.15 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.0; temperature: 293.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Thomas Goddard - data analysis

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker AMX 600 MHz
Varian INOVA 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks