BMRB Entry 25473

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25473

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Title:
Pcf11 second N-terminal domain
Deposition date:
2015-02-06
Original release date:
2016-11-02
Authors:
Xu, Xiaoqian; Mackereth, Cameron
Citation:

Citation: Xu, Xiaoqian; Perebaskine, Natacha; Minvielle-Sebastia, Lionel; Fribourg, Sebastien; Mackereth, Cameron. "Chemical shift assignments of a new folded domain from yeast Pcf11"  Biomol. NMR Assign. 9, 421-425 (2015).
PubMed: 26133941

Assembly members:

Assembly members:
Pcf11, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pcf11: GHMLHQKNLQAMLPTPTVPL LLRDIDKLTCLTSERLKNQP NDEKLKMKLLVLSQLKQELK REKLTLNALKQVQMQLRQVF SQDQQVLQERMRYHEL

Data sets:
Data typeCount
13C chemical shifts454
15N chemical shifts107
1H chemical shifts765

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pcf111

Entities:

Entity 1, Pcf11 96 residues - Formula weight is not available

Contains N-terminal Gly-His-Met residues following cleavage of the His6-tag by TEV protease

1   GLYHISMETLEUHISGLNLYSASNLEUGLN
2   ALAMETLEUPROTHRPROTHRVALPROLEU
3   LEULEUARGASPILEASPLYSLEUTHRCYS
4   LEUTHRSERGLUARGLEULYSASNGLNPRO
5   ASNASPGLULYSLEULYSMETLYSLEULEU
6   VALLEUSERGLNLEULYSGLNGLULEULYS
7   ARGGLULYSLEUTHRLEUASNALALEULYS
8   GLNVALGLNMETGLNLEUARGGLNVALPHE
9   SERGLNASPGLNGLNVALLEUGLNGLUARG
10   METARGTYRHISGLULEU

Samples:

sample_1: Pcf11, [U-99% 15N], 450 uM; sodium phosphate 20 mM; sodium chloride 50 mM; D2O, [U-100% 2H], 10%; H2O 90%; DTT 2 mM

sample_2: Pcf11, [U-99% 13C; U-99% 15N], 200 uM; sodium phosphate 20 mM; sodium chloride 150 mM; D2O, [U-100% 2H], 10%; H2O 90%; DTT 2 mM

sample_3: Pcf11, [U-99% 13C; U-99% 15N], 200 uM; sodium phosphate 20 mM; sodium chloride 150 mM; D2O, [U-100% 2H], 100%; DTT 2 mM

sample_4: Pcf11, [U-10% 13C], 100 uM; sodium phosphate 20 mM; sodium chloride 150 mM; D2O, [U-100% 2H], 10%; H2O 90%; DTT 2 mM

sample_5: Pcf11, [U-13C; U-15N]-Leu, 200 uM; sodium phosphate 20 mM; sodium chloride 150 mM; D2O, [U-100% 2H], 100%; DTT 2 mM

sample_conditions_1: ionic strength: 170 mM; pH: 6.5; pressure: 1 atm; temperature: 300 K

sample_conditions_2: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
3D HCACOsample_2isotropicsample_conditions_1
3D (H)CCONH-TOCSYsample_2isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_3isotropicsample_conditions_1
3D H(C)CH-TOCSYsample_3isotropicsample_conditions_1
2D 1H-13C HSQC (CT)sample_4isotropicsample_conditions_1
3D 1H-15N TOCSYsample_4isotropicsample_conditions_1
3D HCACOsample_5isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_5isotropicsample_conditions_1
3D H(C)CH-TOCSYsample_5isotropicsample_conditions_1
3D HNHAsample_4isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_2
3D 1H-15N NOESYsample_1isotropicsample_conditions_2

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 700 MHz

Related Database Links:

UNP P39081
NCBI NP_010514.3
AlphaFold Q04932

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks