BMRB Entry 25472
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25472
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Kim, Jae hyun; Kim, Jae Il. "Solution Structure of mu-SLPTX-Ssm6a, a Gating Modifier of human Nav1.7 channels" Biochemistry ., .-..
Assembly members:
entity, polymer, 46 residues, 5333.025 Da.
Natural source: Common Name: centipede Taxonomy ID: 55038 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Scolopendra subspinipes
Experimental source: Production method: chemical synthesis Host organism: scolopendra subspinipes mutilans
Entity Sequences (FASTA):
entity: ADNKCENSLRREIACGQCRD
KVKTDGYFYECCTSDSTFKK
CQDLLH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 233 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 46 residues - 5333.025 Da.
| 1 | ALA | ASP | ASN | LYS | CYS | GLU | ASN | SER | LEU | ARG | ||||
| 2 | ARG | GLU | ILE | ALA | CYS | GLY | GLN | CYS | ARG | ASP | ||||
| 3 | LYS | VAL | LYS | THR | ASP | GLY | TYR | PHE | TYR | GLU | ||||
| 4 | CYS | CYS | THR | SER | ASP | SER | THR | PHE | LYS | LYS | ||||
| 5 | CYS | GLN | ASP | LEU | LEU | HIS |
Samples:
sample_1: entity 1.0 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0 M; pH: 3.7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz