BMRB Entry 25456

Title:
Cullin3 - BTB interface: a novel target for stapled peptides
Deposition date:
2015-01-27
Original release date:
2015-04-20
Authors:
Russo, Luigi; Palmieri, Maddalena; Malgieri, Gaetano
Citation:

Citation: de Paola, Ivan; Pirone, Luciano; Palmieri, Maddalena; Balasco, Nicole; Esposito, Luciana; Russo, Luigi; Mazz, Daniela; Di Marcotullio, Lucia; Di Gaetano, Sonia; Malgieri, Gaetano; Vitagliano, Luigi; Pedone, Emilia; Zaccaro, Laura. "Cullin3 - BTB Interface: A Novel Target for Stapled Peptides"  PLoS ONE 10, e0121149-e0121149 (2015).
PubMed: 25848797

Assembly members:

Assembly members:
entity, polymer, 20 residues, 2386.851 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: NSGLSFEXLYRXAYTMVLHK

Data sets:
Data typeCount
1H chemical shifts134

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 20 residues - 2386.851 Da.

1   ASNSERGLYLEUSERPHEGLUMK8LEUTYR
2   ARGMK8ALATYRTHRMETVALLEUHISLYS

Samples:

sample_1: Cul349-68EN 1 mM; trifluoroethanol%; water%

sample_conditions_1: pH: 3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, refinement, structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

PDB