BMRB Entry 25456
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25456
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: de Paola, Ivan; Pirone, Luciano; Palmieri, Maddalena; Balasco, Nicole; Esposito, Luciana; Russo, Luigi; Mazz, Daniela; Di Marcotullio, Lucia; Di Gaetano, Sonia; Malgieri, Gaetano; Vitagliano, Luigi; Pedone, Emilia; Zaccaro, Laura. "Cullin3 - BTB Interface: A Novel Target for Stapled Peptides" PLoS ONE 10, e0121149-e0121149 (2015).
PubMed: 25848797
Assembly members:
entity, polymer, 20 residues, 2386.851 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: NSGLSFEXLYRXAYTMVLHK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 134 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 20 residues - 2386.851 Da.
| 1 | ASN | SER | GLY | LEU | SER | PHE | GLU | MK8 | LEU | TYR | |
| 2 | ARG | MK8 | ALA | TYR | THR | MET | VAL | LEU | HIS | LYS |
Samples:
sample_1: Cul349-68EN 1 mM; trifluoroethanol%; water%
sample_conditions_1: pH: 3; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, refinement, structure solution
NMR spectrometers:
- Varian INOVA 500 MHz
Related Database Links:
| PDB |