BMRB Entry 25445

Name: HN,N,CA,CB assignments of A2A-ct
Published: 2016-06-30

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25445

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

HN,N,CA,CB assignments of A2A-ct

Deposition date:

2015-01-20

Original release date:

2016-06-30

Authors:

Tossavainen, Helena; Hellman, Maarit; Permi, Perttu

Citation:

Citation: Tossavainen, Helena; Hellman, Maarit; Piirainen, Henni; Jaakola, Veli-Pekka; Permi, Perttu. "HN, N, CA and CB assignments of the intrinsically disordered C-terminus of human adenosine A2A receptor" Biomol. NMR Assign. 9, 403-406 (2015).
PubMed: 25952762

Assembly members:

Assembly members:
A2A-ct, polymer, 134 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE-T7-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
A2A-ct: MREFRQTFRKIIRSHVLRQQ EPFKAAGTSARVLAAHGSDG EQVSLRLNGHPPGVWANGSA PHPERRPNGYALGLVSGGSA QESQGNTGLPDVELLSHELK GVCPEPPGLDDPLAQDGAGV SGLEHHHHHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	344
15N chemical shifts	122
1H chemical shifts	111

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	A2A-ct	1

Entities:

Entity 1, A2A-ct 134 residues - Formula weight is not available

1

MET

ARG

GLU

PHE

ARG

GLN

THR

PHE

ARG

LYS

2

ILE

ARG

SER

HIS

VAL

LEU

ARG

GLN

3

GLU

PRO

PHE

LYS

ALA

GLY

THR

SER

ALA

4

ARG

VAL

LEU

ALA

HIS

GLY

SER

ASP

GLY

5

GLU

GLN

VAL

SER

LEU

ARG

LEU

ASN

GLY

HIS

6

PRO

GLY

VAL

TRP

ALA

ASN

GLY

SER

ALA

7

PRO

HIS

PRO

GLU

ARG

PRO

ASN

GLY

TYR

8

ALA

LEU

GLY

LEU

VAL

SER

GLY

SER

ALA

9

GLN

GLU

SER

GLN

GLY

ASN

THR

GLY

LEU

PRO

10

ASP

VAL

GLU

LEU

SER

HIS

GLU

LEU

LYS

11

GLY

VAL

CYS

PRO

GLU

PRO

GLY

LEU

ASP

12

ASP

PRO

LEU

ALA

GLN

ASP

GLY

ALA

GLY

VAL

13

SER

GLY

LEU

GLU

HIS

14

HIS

Samples:

sample_1: A2A-ct, [U-13C; U-15N], 0.6 mM; HEPES 50 mM; TCEP-HCl 1 mM; sodium chloride 250 mM; calcium chloride 5 mM; H2O 95%; D2O, U-2H, 5%

sample_conditions_1: ionic strength: 300 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D i(HCA)CO(CA)NH	sample_1	isotropic	sample_conditions_1
3D (HCA)N(CA)CO(N)H	sample_1	isotropic	sample_conditions_1
3D (HCA)CON(CAN)H	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ, Agilent - processing

SPARKY, Goddard - data analysis

NMR spectrometers:

Agilent INOVA 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks