BMRB Entry 25435

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PDB ID: 2mxx
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25435
MolProbity Validation Chart

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Title:
Structure of Amylase binding Protein A of Streptococcous gordonii: a potential receptor for human salivary amylase enzyme
Deposition date:
2015-01-18
Original release date:
2015-05-11
Authors:
Sethi, Ashish; Mohanty, Biswaranjan; Ramasubbu, Narayanan; Gooley, Paul
Citation:

Citation: Sethi, Ashish; Mohanty, Biswaranjan; Ramasubbu, Narayanan; Gooley, Paul. "Structure of amylase-binding protein A of Streptococcus gordonii: A potential receptor for human salivary alpha-amylase enzyme"  Protein Sci. 24, 1013-1018 (2015).
PubMed: 25739638

Assembly members:

Assembly members:
entity, polymer, 179 residues, 19170.961 Da.

Natural source:

Natural source:   Common Name: firmicutes   Taxonomy ID: 29390   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus gordonii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: E. coli BL21 (DE3) bacteria   Vector: pET29b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MADEATDAARNNDGAYYLQT QFTNADKVNEYLAQHDGEIR AEAAADPAVVAAKAALDAVE GGSHNYGEVKAAYEAAFNNA FNAVRNKYVQRFQATYNNAT EQEGKTYIQGETPEQANARY LKRVGAANNQNPAAEDKGAT TPASKEEAKKSEAAAKNAGK AAGKALPKTSAVKHHHHHH

Data sets:
Data typeCount
13C chemical shifts640
15N chemical shifts177
1H chemical shifts991

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 179 residues - 19170.961 Da.

1   METALAASPGLUALATHRASPALAALAARG
2   ASNASNASPGLYALATYRTYRLEUGLNTHR
3   GLNPHETHRASNALAASPLYSVALASNGLU
4   TYRLEUALAGLNHISASPGLYGLUILEARG
5   ALAGLUALAALAALAASPPROALAVALVAL
6   ALAALALYSALAALALEUASPALAVALGLU
7   GLYGLYSERHISASNTYRGLYGLUVALLYS
8   ALAALATYRGLUALAALAPHEASNASNALA
9   PHEASNALAVALARGASNLYSTYRVALGLN
10   ARGPHEGLNALATHRTYRASNASNALATHR
11   GLUGLNGLUGLYLYSTHRTYRILEGLNGLY
12   GLUTHRPROGLUGLNALAASNALAARGTYR
13   LEULYSARGVALGLYALAALAASNASNGLN
14   ASNPROALAALAGLUASPLYSGLYALATHR
15   THRPROALASERLYSGLUGLUALALYSLYS
16   SERGLUALAALAALALYSASNALAGLYLYS
17   ALAALAGLYLYSALALEUPROLYSTHRSER
18   ALAVALLYSHISHISHISHISHISHIS

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