BMRB Entry 25426

Title:
MBD2 intrinsically disordered region
Deposition date:
2015-01-12
Original release date:
2018-02-23
Authors:
Williams, David
Citation:

Citation: Desai, Megha; Webb, Heather; Sinanan, Leander; Scarsdale, Neel; Walavalkar, Ninad; Ginder, Gordon; Williams, David. "An intrinsically disordered region of methyl-CpG binding domain protein 2 (MBD2) recruits the histone deacetylase core of the NuRD complex"  Nucleic Acids Res. 43, 3100-3113 (2015).
PubMed: 25753662

Assembly members:

Assembly members:
MBD2, polymer, 121 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet32a

Data sets:
Data typeCount
13C chemical shifts331
15N chemical shifts106
1H chemical shifts366

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MBD2 IDR1

Entities:

Entity 1, MBD2 IDR 121 residues - Formula weight is not available

Residue 1-2 represent residual from non-native affinity tag.

1   GLYSERASPLEUASNTHRTHRLEUPROILE
2   ARGGLNTHRALASERILEPHELYSGLNPRO
3   VALTHRLYSVALTHRASNHISPROSERASN
4   LYSVALLYSSERASPPROGLNARGMETASN
5   GLUGLNPROARGGLNLEUPHETRPGLULYS
6   ARGLEUGLNGLYLEUSERALASERASPVAL
7   THRGLUGLNILEILELYSTHRMETGLULEU
8   PROLYSGLYLEUGLNGLYVALGLYPROGLY
9   SERASNASPGLUTHRLEULEUSERALAVAL
10   ALASERALALEUHISTHRSERSERALAPRO
11   ILETHRGLYGLNVALSERALAALAVALGLU
12   LYSASNPROALAVALTRPLEUASNTHRSER
13   GLN

Samples:

sample_1: MBD2, [U-99% 13C; U-99% 15N], 0.5 – 1 mM; H2O 90%; D2O 10%; NaH2PO4 10 mM; sodium azide 0.02%; DTT 1 mM

sample_conditions_1: ionic strength: 25 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CcpNMR, CCPN - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks