BMRB Entry 25415

Name: Structural features of a 3&#039; splice site influenza A: 19-nt duplex
Published: 2015-05-04

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PDB ID: 2mxk
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25415
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structural features of a 3' splice site influenza A: 19-nt duplex

Deposition date:

2015-01-06

Original release date:

2015-05-04

Authors:

Turner, Douglas; Kennedy, Scott; Chen, Jonathan

Citation:

Citation: Chen, Jonathan; Kennedy, Scott; Turner, Douglas. "Structural features of a 3' splice site in influenza A" Biochemistry 54, 3269-3285 (2015).
PubMed: 25909229

Assembly members:

Assembly members:
RNA_(19-MER), polymer, 19 residues, 6171.794 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA_(19-MER): GCAGGCCCAUGGGAGUGCA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	140
31P chemical shifts	14

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity	1

Entities:

Entity 1, entity 19 residues - 6171.794 Da.

1

G

C

A

G

C

A

U

2

G

A

G

U

G

C

A

Samples:

sample_1: entity 4.0 mM; H2O 95%; D2O 5%

sample_2: entity 1.0 mM; D2O 100%

sample_conditions_1: ionic strength: 101.12 mM; pH: 6.0; pressure: 1 atm; temperature: 298.15 K

sample_conditions_2: ionic strength: 101.12 mM; pH: 6.0; pressure: 1 atm; temperature: 273.15 K

sample_conditions_3: ionic strength: 101.12 mM; pH: 6.0; pressure: 1 atm; temperature: 271.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_2
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_3
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_3
2D 1H-31P HETCOR	sample_2	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_2

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMR, Varian - collection

NMR spectrometers:

Varian INOVA 600 MHz
Varian INOVA 500 MHz