BMRB Entry 25410

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR25410

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Title:
Backbone assignments of a construct of the Sigma-1 receptor spanning residues 35-223
Deposition date:
2015-01-04
Original release date:
2016-06-30
Authors:
Ortega-Roldan, Jose L.; Schnell, Jason
Citation:

Citation: Ortega-Roldan, Jose Luis; Ossa, Felipe; Amin, Nader; Schnell, Jason. "Solution NMR studies reveal the location of the second transmembrane domain of the human sigma-1 receptor"  FEBS Lett. 589, 659-665 (2015).
PubMed: 25647032

Assembly members:

Assembly members:
Sigma-1_Receptor_delta35, polymer, 275 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pCold-I

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sigma-1_Receptor_delta35: MNHKVHHHHHHIEGRHMFVF QREEIAQLARQYAGLDHELA FSRLIVELRRLHPGHVLPDE ELQWVFVNAGGWMGAMALLH ASLSEYVLLFGTALGSRGHS GRYWAEISDTIISGTFHQWR EGTTKSEVFYPGETVVHGPG EATAVEWGPNTWMVEYGRGV IPSTLAFALADTVFSTQDFL TLFYTLRSYARGLRLELTTY LFGQDPGPGEATAVEWGPNT WMVEYGRGVIPSTLAFALAD TVFSTQDFLTLFYTLRSYAR GLRLELTTYLFGQDP

Data sets:
Data typeCount
13C chemical shifts429
15N chemical shifts169
1H chemical shifts168

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sigma-1 receptor delta 35 construct1

Entities:

Entity 1, Sigma-1 receptor delta 35 construct 275 residues - Formula weight is not available

1   METASNHISLYSVALHISHISHISHISHIS
2   HISILEGLUGLYARGHISMETPHEVALPHE
3   GLNARGGLUGLUILEALAGLNLEUALAARG
4   GLNTYRALAGLYLEUASPHISGLULEUALA
5   PHESERARGLEUILEVALGLULEUARGARG
6   LEUHISPROGLYHISVALLEUPROASPGLU
7   GLULEUGLNTRPVALPHEVALASNALAGLY
8   GLYTRPMETGLYALAMETALALEULEUHIS
9   ALASERLEUSERGLUTYRVALLEULEUPHE
10   GLYTHRALALEUGLYSERARGGLYHISSER
11   GLYARGTYRTRPALAGLUILESERASPTHR
12   ILEILESERGLYTHRPHEHISGLNTRPARG
13   GLUGLYTHRTHRLYSSERGLUVALPHETYR
14   PROGLYGLUTHRVALVALHISGLYPROGLY
15   GLUALATHRALAVALGLUTRPGLYPROASN
16   THRTRPMETVALGLUTYRGLYARGGLYVAL
17   ILEPROSERTHRLEUALAPHEALALEUALA
18   ASPTHRVALPHESERTHRGLNASPPHELEU
19   THRLEUPHETYRTHRLEUARGSERTYRALA
20   ARGGLYLEUARGLEUGLULEUTHRTHRTYR
21   LEUPHEGLYGLNASPPROGLYPROGLYGLU
22   ALATHRALAVALGLUTRPGLYPROASNTHR
23   TRPMETVALGLUTYRGLYARGGLYVALILE
24   PROSERTHRLEUALAPHEALALEUALAASP
25   THRVALPHESERTHRGLNASPPHELEUTHR
26   LEUPHETYRTHRLEUARGSERTYRALAARG
27   GLYLEUARGLEUGLULEUTHRTHRTYRLEU
28   PHEGLYGLNASPPRO

Samples:

S1chtriple: Sigma-1 Receptor delta35, [U-100% 13C; U-100% 15N; U-80% 2H], 0.27 mM; H2O 95%; D2O 5%

S1chdoble: Sigma-1 Receptor delta35, [U-100% 13C; U-100% 15N], 0.27 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0 M; pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCS1chtripleisotropicsample_conditions_1
3D HNCAS1chtripleisotropicsample_conditions_1
3D HNCACBS1chtripleisotropicsample_conditions_1
3D 1H-15N TOCSYS1chdobleisotropicsample_conditions_1
3D 1H-15N NOESYS1chdobleisotropicsample_conditions_1
3D HNCOS1chtripleisotropicsample_conditions_1
3D CBCA(CO)NHS1chtripleisotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks