BMRB Entry 25402

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25402
MolProbity Validation Chart

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Title:
Solution structure of the full length sorting nexin 3
Deposition date:
2014-12-20
Original release date:
2015-08-13
Authors:
Lenoir, Marc; Rajesh, Sandya; Gruenberg, Jean; Overduin, Michael; Kaur, Jaswant
Citation:

Citation: Overduin, Michael; Rajesh, Sandya; Gruenberg, Jean; Lenoir, Marc. "Secondary structure and 1H, 13C, 15N resonance assignments of the endosomal sorting protein sorting nexin 3"  Biomol. NMR Assign. 9, 355-358 (2015).
PubMed: 25893673

Assembly members:

Assembly members:
SNX3FL, polymer, 172 residues, 19924.6031 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SNX3FL: MAHHHHHHVGTAETVADTRR LITKPQNLNDAYGPPSNFLE IDVSNPQTVGVGRGRFTTYE IRVKTNLPIFKLKESTVRRR YSDFEWLRSELERESKVVVP PLPGKAFLRQLPFRGDDGIF DDNFIEERKQGLEQFINKVA GHPLAQNERCLHMFLQDEII DKSYTPSKIRHA

Data sets:
Data typeCount
13C chemical shifts729
15N chemical shifts165
1H chemical shifts1177

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SNX3FL1

Entities:

Entity 1, SNX3FL 172 residues - 19924.6031 Da.

1   METALAHISHISHISHISHISHISVALGLY
2   THRALAGLUTHRVALALAASPTHRARGARG
3   LEUILETHRLYSPROGLNASNLEUASNASP
4   ALATYRGLYPROPROSERASNPHELEUGLU
5   ILEASPVALSERASNPROGLNTHRVALGLY
6   VALGLYARGGLYARGPHETHRTHRTYRGLU
7   ILEARGVALLYSTHRASNLEUPROILEPHE
8   LYSLEULYSGLUSERTHRVALARGARGARG
9   TYRSERASPPHEGLUTRPLEUARGSERGLU
10   LEUGLUARGGLUSERLYSVALVALVALPRO
11   PROLEUPROGLYLYSALAPHELEUARGGLN
12   LEUPROPHEARGGLYASPASPGLYILEPHE
13   ASPASPASNPHEILEGLUGLUARGLYSGLN
14   GLYLEUGLUGLNPHEILEASNLYSVALALA
15   GLYHISPROLEUALAGLNASNGLUARGCYS
16   LEUHISMETPHELEUGLNASPGLUILEILE
17   ASPLYSSERTYRTHRPROSERLYSILEARG
18   HISALA

Samples:

Assignments: SNX3FL, [U-100% 13C; U-100% 15N], 0.5 mM; NaCl 100 mM; Sodium phosphate 20 mM

CondSet1: ionic strength: 0.100 M; pH: 6.5; pressure: 1.000 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCAssignmentsisotropicCondSet1
3D HNCAAssignmentsisotropicCondSet1
3D HN(CO)CAAssignmentsisotropicCondSet1
3D HNCOAssignmentsisotropicCondSet1
3D CBCA(CO)NHAssignmentsisotropicCondSet1
3D HNCACBAssignmentsisotropicCondSet1
3D HNCOAssignmentsisotropicCondSet1
3D HN(CA)COAssignmentsisotropicCondSet1
3D HCCH-TOCSYAssignmentsisotropicCondSet1
3D 1H-13C NOESYAssignmentsisotropicCondSet1
3D 1H-15N NOESYAssignmentsisotropicCondSet1
2D 1H-13C HSQCAssignmentsisotropicCondSet1

Software:

CcpNmr_Analysis v2.2, CCPN, Linge, O'Donoghue and Nilges - chemical shift calculation, data analysis, structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian UnityInova 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks