BMRB Entry 25401

Name: 1H, 15N 13C Chemical shift assignments for CCL28
Published: 2015-01-20

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25401

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N 13C Chemical shift assignments for CCL28

Deposition date:

2014-12-19

Original release date:

2015-01-20

Authors:

Thomas, Monica; Peterson, Francis; Volkman, Brian

Citation:

Citation: Thomas, Monica; Buelow, Becky; Nevins, Amanda; Jones, Stephanie; Peterson, Francis; Gundry, Rebekah; Grayson, Mitchell; Volkman, Brian. "Structure-function analysis of CCL28 in post viral asthma" J. Biol. Chem. ., .-..

Assembly members:

Assembly members:
CCL28, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CCL28: SEAILPIASSCCTEVSHHIS RRLLERVNMCRIQRADGDCD LAAVILHVKRRRICVSPHNH TVKQWMKVQAAKKNGKGNVC HRKKHHGKRNSNRAHQGKHE TYGHKTPY

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	434
1H chemical shifts	698
15N chemical shifts	110

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CCL28	1

Entities:

Entity 1, CCL28 108 residues - Formula weight is not available

1

SER

GLU

ALA

ILE

LEU

PRO

ILE

ALA

SER

2

CYS

THR

GLU

VAL

SER

HIS

ILE

SER

3

ARG

LEU

GLU

ARG

VAL

ASN

MET

CYS

4

ARG

ILE

GLN

ARG

ALA

ASP

GLY

ASP

CYS

ASP

5

LEU

ALA

VAL

ILE

LEU

HIS

VAL

LYS

ARG

6

ARG

ILE

CYS

VAL

SER

PRO

HIS

ASN

HIS

7

THR

VAL

LYS

GLN

TRP

MET

LYS

VAL

GLN

ALA

8

ALA

LYS

ASN

GLY

LYS

GLY

ASN

VAL

CYS

9

HIS

ARG

LYS

HIS

GLY

LYS

ARG

ASN

10

SER

ASN

ARG

ALA

HIS

GLN

GLY

LYS

HIS

GLU

11

THR

TYR

GLY

HIS

LYS

THR

PRO

TYR

Samples:

sample_1: CCL28, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 20 mM; sodium azide 0.02%; D2O, [U-99% 2H], 10%

sample_conditions_1: temperature: 298 K; pH: 6.2; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - data analysis, chemical shift assignment

NMR spectrometers:

Bruker DRX 600 MHz

DBJ	BAG35056 BAJ20648
GB	AAF87205 AAG16691 AAG43193 AAH62668 AAH69532
REF	NP_001288802 NP_001288803 NP_001288804 NP_683513 XP_001135733
SP	Q9NRJ3
AlphaFold	Q9NRJ3