BMRB Entry 25393

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for IST1 residues 303-366
Published: 2015-05-26

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25393

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for IST1 residues 303-366

Deposition date:

2014-12-15

Original release date:

2015-05-26

Authors:

Wenzel, Dawn; Skalicky, Jack; Sundquist, Wesley

Citation:

Citation: Caballe, Anna; Wenzel, Dawn; Agromayor, Monica; Alam, Steven; Skalicky, Jack; Kloc, Magdalena; Carlton, Jeremy; Labrador, Leticia; Sundquist, Wesley; Martin-Serrano, Juan. "ULK3 regulates cytokinetic abscission by phosphorylating ESCRT-III proteins" Elife 4, e06547-e06547 (2015).
PubMed: 26011858

Assembly members:

Assembly members:
IST1, polymer, 67 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IST1: GHMGPKPEASAKLPSRPADN YDNFVLPELPSVPDTLPTAS AGASTSASEDIDFDDLSRRF EELKKKT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	189
15N chemical shifts	56
1H chemical shifts	56

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	IST1 peptide	1

Entities:

Entity 1, IST1 peptide 67 residues - Formula weight is not available

1

GLY

HIS

MET

GLY

PRO

LYS

PRO

GLU

ALA

SER

2

ALA

LYS

LEU

PRO

SER

ARG

PRO

ALA

ASP

ASN

3

TYR

ASP

ASN

PHE

VAL

LEU

PRO

GLU

LEU

PRO

4

SER

VAL

PRO

ASP

THR

LEU

PRO

THR

ALA

SER

5

ALA

GLY

ALA

SER

THR

SER

ALA

SER

GLU

ASP

6

ILE

ASP

PHE

ASP

LEU

SER

ARG

PHE

7

GLU

LEU

LYS

THR

Samples:

sample_1: IST1, [U-13C; U-15N], 0.4 mM; sodium phosphate 20 mM; NaCl 25 mM; EDTA 0.1 mM; DTT 0.5 mM; sodium azide 0.1 mM

sample_conditions_1: ionic strength: 0.025 M; pH: 6.2; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ, Varian - collection

NMR spectrometers:

Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks