BMRB Entry 25385
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25385
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Citation: Hu, Wanhui; Wu, Huiwen; Zhang, Hong; Gong, Weibin; Perrett, Sarah. "Resonance assignments for the substrate binding domain of Hsp70 chaperone Ssa1 from Saccharomyces cerevisiae" Biomol. NMR Assign. 9, 329-332 (2015).
PubMed: 25682100
Assembly members:
Ssa1_substrate_binding_domain, polymer, 174 residues, 18838.3 Da.
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eucaryotes Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
Ssa1_substrate_binding_domain: SSSKTQDLLLLDVAPLSLGI
ETAGGVMTKLIPRNSTIPTK
KSEIFSTYADNQPGVLIQVF
EGERAKTKDNNLLGKFELSG
IPPAPRGVPQIEVTFDVDSN
GILNVSAVEKGTGKSNKITI
TNDKGRLSKEDIEKMVAEAE
KFKEEDEKESQRIASKNQLE
SIAYSLKNTISEAG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 727 |
| 15N chemical shifts | 176 |
| 1H chemical shifts | 1109 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ssa1 substrate binding domain | 1 |
Entities:
Entity 1, Ssa1 substrate binding domain 174 residues - 18838.3 Da.
| 1 | SER | SER | SER | LYS | THR | GLN | ASP | LEU | LEU | LEU | ||||
| 2 | LEU | ASP | VAL | ALA | PRO | LEU | SER | LEU | GLY | ILE | ||||
| 3 | GLU | THR | ALA | GLY | GLY | VAL | MET | THR | LYS | LEU | ||||
| 4 | ILE | PRO | ARG | ASN | SER | THR | ILE | PRO | THR | LYS | ||||
| 5 | LYS | SER | GLU | ILE | PHE | SER | THR | TYR | ALA | ASP | ||||
| 6 | ASN | GLN | PRO | GLY | VAL | LEU | ILE | GLN | VAL | PHE | ||||
| 7 | GLU | GLY | GLU | ARG | ALA | LYS | THR | LYS | ASP | ASN | ||||
| 8 | ASN | LEU | LEU | GLY | LYS | PHE | GLU | LEU | SER | GLY | ||||
| 9 | ILE | PRO | PRO | ALA | PRO | ARG | GLY | VAL | PRO | GLN | ||||
| 10 | ILE | GLU | VAL | THR | PHE | ASP | VAL | ASP | SER | ASN | ||||
| 11 | GLY | ILE | LEU | ASN | VAL | SER | ALA | VAL | GLU | LYS | ||||
| 12 | GLY | THR | GLY | LYS | SER | ASN | LYS | ILE | THR | ILE | ||||
| 13 | THR | ASN | ASP | LYS | GLY | ARG | LEU | SER | LYS | GLU | ||||
| 14 | ASP | ILE | GLU | LYS | MET | VAL | ALA | GLU | ALA | GLU | ||||
| 15 | LYS | PHE | LYS | GLU | GLU | ASP | GLU | LYS | GLU | SER | ||||
| 16 | GLN | ARG | ILE | ALA | SER | LYS | ASN | GLN | LEU | GLU | ||||
| 17 | SER | ILE | ALA | TYR | SER | LEU | LYS | ASN | THR | ILE | ||||
| 18 | SER | GLU | ALA | GLY |
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