BMRB Entry 25384

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25384

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Title:
The Beclin 1 N-terminal Domain (BecN-150CSY)
Deposition date:
2014-12-08
Original release date:
2016-06-30
Authors:
Yao, Shenggen; Lee, Erinna; Fairlie, Walter; Keizer, David
Citation:

Citation: Yao, Shenggen; Lee, Erinna; Pettikiriarachchi, Anne; Evangelista, Marco; Keizer, David; Fairlie, Walter. "Characterisation of the conformational preference and dynamics of the intrinsically disordered N-terminal region of Beclin 1 by NMR spectroscopy"  Biochim. Biophys. Acta 1864, 1128-1137 (2016).

Assembly members:

Assembly members:
BecN-150CSY, polymer, 165 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET DUET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BecN-150CSY: MGSSHHHHHHSQDPMEGSKT SNNSTMQVSFVSQRSSQPLK LDTSFKILDRVTIQELTAPL LTTAQAKPGETQEEETNSGE EPFIETPRQDGVSRRFIPPA RMMSTESANSFTLIGEASDG GTMENLSRRLKVTGDLFDIM SGQTDVDHPLSEESTDTLLD QLDTY

Data sets:
Data typeCount
13C chemical shifts438
15N chemical shifts142
1H chemical shifts293

Additional metadata:

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  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 165 residues - Formula weight is not available

Residues (-14) - (-1) represent a non-native tag Residue 151 is non-native added to facilitate quantitation by UV spectroscopy

1   METGLYSERSERHISHISHISHISHISHIS
2   SERGLNASPPROMETGLUGLYSERLYSTHR
3   SERASNASNSERTHRMETGLNVALSERPHE
4   VALSERGLNARGSERSERGLNPROLEULYS
5   LEUASPTHRSERPHELYSILELEUASPARG
6   VALTHRILEGLNGLULEUTHRALAPROLEU
7   LEUTHRTHRALAGLNALALYSPROGLYGLU
8   THRGLNGLUGLUGLUTHRASNSERGLYGLU
9   GLUPROPHEILEGLUTHRPROARGGLNASP
10   GLYVALSERARGARGPHEILEPROPROALA
11   ARGMETMETSERTHRGLUSERALAASNSER
12   PHETHRLEUILEGLYGLUALASERASPGLY
13   GLYTHRMETGLUASNLEUSERARGARGLEU
14   LYSVALTHRGLYASPLEUPHEASPILEMET
15   SERGLYGLNTHRASPVALASPHISPROLEU
16   SERGLUGLUSERTHRASPTHRLEULEUASP
17   GLNLEUASPTHRTYR

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