BMRB Entry 25371

Title:
NMR assignments of a novel lectin from sea mussel Crenomytilus grayanus
Deposition date:
2014-12-01
Original release date:
2022-05-12
Authors:
Chien, Chih-Ta Henry; Hsu, Shang-Te Danny
Citation:

Citation: Liao, Jiahn-Haur; Chien, Chih-Ta Henry; Wu, Han-Ying; Huang, Kai-Fa; Wang, Iren; Ho, Meng-Ru; Tu, I-Fan; Lee, I-Ming; Li, Wei; Shih, Yu-Ling; Wu, Chung-Yi; Lukyanov, Pavel; Hsu, Shang-Te Danny; Wu, Shih-Hsiung. "A Multivalent Marine Lectin from Crenomytilus grayanus Possesses Anti-cancer Activity through Recognizing Globotriose Gb3"  J. Am. Chem. Soc. 138, 4787-4795 (2016).
PubMed: 27010847

Assembly members:

Assembly members:
CG-lectin, polymer, 156 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: bivalves   Taxonomy ID: 151218   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Crenomytilus grayanus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Data sets:
Data typeCount
13C chemical shifts542
15N chemical shifts147
1H chemical shifts882

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CGL1

Entities:

Entity 1, CGL 156 residues - Formula weight is not available

1   METTHRTHRPHELEUILELYSHISLYSALA
2   SERGLYLYSPHELEUHISPROTYRGLYGLY
3   SERSERASNPROALAASNASNTHRLYSLEU
4   VALLEUHISSERASPILEHISGLUARGMET
5   TYRPHEGLNPHEASPVALVALASPGLUARG
6   TRPGLYTYRILELYSHISVALALASERGLY
7   LYSILEVALHISPROTYRGLYGLYGLNALA
8   ASNPROPROASNGLUTHRASNMETVALLEU
9   HISGLNASPARGHISASPARGALALEUPHE
10   ALAMETASPPHEPHEASNASPASNILEMET
11   HISLYSGLYGLYLYSTYRILEHISPROLYS
12   GLYGLYSERPROASNPROPROASNASNTHR
13   GLUTHRVALILEHISGLYASPLYSHISALA
14   ALAMETGLUPHEILEPHEVALSERPROLYS
15   ASNLYSASPLYSARGVALLEUVALTYRALA
16   HISHISHISHISHISHIS

Samples:

sample_1: CG-lectin, [U-100% 13C; U-100% 15N], 400 uM; H20 90%; D2O 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks