BMRB Entry 25355

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25355

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
EphB2 kinase domain and juxtamembrane segment, S677A/S680A/D754A
Deposition date:
2014-11-20
Original release date:
2014-12-19
Authors:
Lundstrom, Patrik; Ahlner, Alexandra
Citation:

Citation: Niklasson, Markus; Ahlner, Alexandra; Andresen, Cecilia; Marsh, Joseph; Lundstrom, Patrik. "Fast and accurate resonance assignment of small-to-large proteins by combining automated and manual approaches"  PLOS Comput. Biol. 11, e1004022-e1004022 (2015).
PubMed: 25569628

Assembly members:

Assembly members:
EphB2_kinase_domain, polymer, 310 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: House mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: N.A.

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EphB2_kinase_domain: MTPGMKIYIDPFTYEDPNEA VREFAKEIDISCVKIEQVIG AGEFGEVCSGHLKLPGKREI FVAIKTLKSGYTEKQRRDFL AEAAIMGQFDHPNVIHLEGV VTKSTPVMIITEFMENGSLD SFLRQNDGQFTVIQLVGMLR GIAAGMKYLADMNYVHRDLA ARNILVNSNLVCKVSDFGLS RFLEDDTSDPTYTSALGGKI PIRWTAPEAIQYRKFTSASD VWSYGIVMWEVMSYGERPYW DMTNQDVINAIEQDYRLPPP MDCPSALHQLMLDCWQKDRN HRPKFGQIVNTLDKMIRNPN SLKAMAPLSS

Data sets:
Data typeCount
13C chemical shifts799
15N chemical shifts240
1H chemical shifts240

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EphB2 kinase domain1

Entities:

Entity 1, EphB2 kinase domain 310 residues - Formula weight is not available

1   METTHRPROGLYMETLYSILETYRILEASP
2   PROPHETHRTYRGLUASPPROASNGLUALA
3   VALARGGLUPHEALALYSGLUILEASPILE
4   SERCYSVALLYSILEGLUGLNVALILEGLY
5   ALAGLYGLUPHEGLYGLUVALCYSSERGLY
6   HISLEULYSLEUPROGLYLYSARGGLUILE
7   PHEVALALAILELYSTHRLEULYSSERGLY
8   TYRTHRGLULYSGLNARGARGASPPHELEU
9   ALAGLUALAALAILEMETGLYGLNPHEASP
10   HISPROASNVALILEHISLEUGLUGLYVAL
11   VALTHRLYSSERTHRPROVALMETILEILE
12   THRGLUPHEMETGLUASNGLYSERLEUASP
13   SERPHELEUARGGLNASNASPGLYGLNPHE
14   THRVALILEGLNLEUVALGLYMETLEUARG
15   GLYILEALAALAGLYMETLYSTYRLEUALA
16   ASPMETASNTYRVALHISARGASPLEUALA
17   ALAARGASNILELEUVALASNSERASNLEU
18   VALCYSLYSVALSERASPPHEGLYLEUSER
19   ARGPHELEUGLUASPASPTHRSERASPPRO
20   THRTYRTHRSERALALEUGLYGLYLYSILE
21   PROILEARGTRPTHRALAPROGLUALAILE
22   GLNTYRARGLYSPHETHRSERALASERASP
23   VALTRPSERTYRGLYILEVALMETTRPGLU
24   VALMETSERTYRGLYGLUARGPROTYRTRP
25   ASPMETTHRASNGLNASPVALILEASNALA
26   ILEGLUGLNASPTYRARGLEUPROPROPRO
27   METASPCYSPROSERALALEUHISGLNLEU
28   METLEUASPCYSTRPGLNLYSASPARGASN
29   HISARGPROLYSPHEGLYGLNILEVALASN
30   THRLEUASPLYSMETILEARGASNPROASN
31   SERLEULYSALAMETALAPROLEUSERSER

Samples:

sample_1: EphB2 kinase domain, [U-100% 13C; U-100% 15N; U-80% 2H], 0.6 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HN(CA)CBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNOsample_1isotropicsample_conditions_1

Software:

COMPASS, Niklasson, Ahlner, Andresen, Marsh, Lundstrom - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA03537 BAA06506 BAA07073 BAE06100 BAG10257
EMBL CAA53598 CAA62862
GB AAA48667 AAA72411 AAA74244 AAA99310 AAB30284
PIR I48760
PRF 2109312A 2124353A
REF NP_001120791 NP_001178427 NP_001277682 NP_001296121 NP_001296122
SP P28693 P29323 P54763 Q90344
TPG DAA32240
AlphaFold P28693 P29323 P54763 Q90344

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks