BMRB Entry 25354

Name: SH3 domain from yeast Abp1p
Published: 2014-12-19

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25354

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SH3 domain from yeast Abp1p

Deposition date:

2014-11-20

Original release date:

2014-12-19

Authors:

Lundstrom, Patrik

Citation:

Citation: Niklasson, Markus; Ahlner, Alexandra; Andresen, Cecilia; Marsh, Joseph; Lundstrom, Patrik. "Fast and accurate resonance assignment of small-to-large proteins by combining automated and manual approaches" PLOS Comput. Biol. 11, e1004022-e1004022 (2015).
PubMed: 25569628

Assembly members:

Assembly members:
Abp1p_SH3, polymer, 62 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: N.A.

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Abp1p_SH3: GAMAPWATAEYDYDAAEDNE LTFVENDKIINIEFVDDDWW LGELEKDGSKGLFPSNYVSL GN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	215
15N chemical shifts	57
1H chemical shifts	311

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Abp1p SH3 domain	1

Entities:

Entity 1, Abp1p SH3 domain 62 residues - Formula weight is not available

1

GLY

ALA

MET

ALA

PRO

TRP

ALA

THR

ALA

GLU

2

TYR

ASP

TYR

ASP

ALA

GLU

ASP

ASN

GLU

3

LEU

THR

PHE

VAL

GLU

ASN

ASP

LYS

ILE

4

ASN

ILE

GLU

PHE

VAL

ASP

TRP

5

LEU

GLY

GLU

LEU

GLU

LYS

ASP

GLY

SER

LYS

6

GLY

LEU

PHE

PRO

SER

ASN

TYR

VAL

SER

LEU

7

GLY

ASN

Samples:

sample_1: Abp1p SH3, [U-13C; U-15N], 0.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

COMPASS, Niklasson, Ahlner, Andresen, Marsh, Lundstrom - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	16843 18054 18055 18056 18057 18058 18059 18060 18061 18062 18063 18064 18065 18066 18067 18068 18069 18070 18071 18072 18073 18074 18075 18076 18077 18078 26519
PDB	1JO8 2K3B 2RPN
DBJ	GAA21988
EMBL	CAA36075 CAA42253 CAY78291
GB	AHN96147 AHV79349 AHY79755 AJP37506 AJQ31991
PRF	1603360A
REF	NP_010012
SP	P15891
TPG	DAA07557
AlphaFold	P15891