BMRB Entry 25351

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25351

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Title:
1H, 13C and 15N Chemical Shift Assignments of the H962C mutant from Arkadia (RNF111) E3 RING domain
Deposition date:
2014-11-19
Original release date:
2019-07-11
Authors:
Marousis, Konstantinos; Tsapardoni, Stamatina; Birkou, Maria; Chasapis, Christos; Bentrop, Detlef; Spyroulias, Georgios
Citation:

Citation: Birkou, Maria; Chasapis, Christos; Marousis, Konstantinos; Loutsidou, Ariadni; Bentrop, Detlef; Lelli, Moreno; Herrmann, Torsten; Carthy, Jonathon; Episkopou, Vasso; Spyroulias, Georgios. "A Residue Specific Insight into the Arkadia E3 Ubiquitin Ligase Activity and Conformational Plasticity"  J. Mol. Biol. 429, 2373-2386 (2017).
PubMed: 28647409

Assembly members:

Assembly members:
entity, polymer, 69 residues, 7917 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MKQDGEEGTEEDTEEKCTIC LSILEEGEDVRRLPCMCLFH QVCVDQWLITNKKCPICRVD IEAQLPSES

Data sets:
Data typeCount
13C chemical shifts291
15N chemical shifts65
1H chemical shifts444

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1
2zinc ion, 12
3zinc ion, 22

Entities:

Entity 1, entity 69 residues - 7917 Da.

1   METLYSGLNASPGLYGLUGLUGLYTHRGLU
2   GLUASPTHRGLUGLULYSCYSTHRILECYS
3   LEUSERILELEUGLUGLUGLYGLUASPVAL
4   ARGARGLEUPROCYSMETCYSLEUPHEHIS
5   GLNVALCYSVALASPGLNTRPLEUILETHR
6   ASNLYSLYSCYSPROILECYSARGVALASP
7   ILEGLUALAGLNLEUPROSERGLUSER

Entity 2, zinc ion, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: H962C, [U-99% 15N], 0.40 mM; H2O 90%; D2O 10%

sample_2: H962C, [U-98% 13C; U-98% 15N], 0.30 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HCACOsample_2isotropicsample_conditions_1
3D HN(COCA)CBsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1

Software:

CARA v1.5.5, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz

Related Database Links:

UNP Q6ZNA4
AlphaFold Q9NSR1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks