BMRB Entry 25333

Name: BILE SALT RECOGNITION BY HUMAN LIVER FATTY ACID BINDING PROTEIN
Published: 2015-08-25

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25333

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

BILE SALT RECOGNITION BY HUMAN LIVER FATTY ACID BINDING PROTEIN

Deposition date:

2014-11-12

Original release date:

2015-08-25

Authors:

Favretto, Filippo; Santambrogio, Carlo; D'Onofrio, Mariapina; Molinari, Henriette; Grandori, Rita; Assfalg, Michael

Citation:

Citation: Favretto, Filippo; Santambrogio, Carlo; D'Onofrio, Mariapina; Molinari, Henriette; Grandori, Rita; Assfalg, Michael. "Bile salt recognition by human liver fatty acid binding protein" FEBS J. 282, 1271-1288 (2015).
PubMed: 25639618

Assembly members:

Assembly members:
hL-FABP, polymer, 135 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE50

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hL-FABP: MRGSMSFSGKYQLQSQENFE AFMKAIGLPEELIQKGKDIK GVSEIVQNGKHFKFTITAGS KVIQNEFTVGEECELETMTG EKVKTVVQLEGDNKLVTAFK NIKSVTELNGDIITNTMTLG DIVFKRISKRILVPR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	250
15N chemical shifts	130
1H chemical shifts	130

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	human liver fatty acid binding protein	1

Entities:

Entity 1, human liver fatty acid binding protein 135 residues - Formula weight is not available

First and last four residues are added for cloning and tag purposes. Variant T94A

1

MET

ARG

GLY

SER

MET

SER

PHE

SER

GLY

LYS

2

TYR

GLN

LEU

GLN

SER

GLN

GLU

ASN

PHE

GLU

3

ALA

PHE

MET

LYS

ALA

ILE

GLY

LEU

PRO

GLU

4

GLU

LEU

ILE

GLN

LYS

GLY

LYS

ASP

ILE

LYS

5

GLY

VAL

SER

GLU

ILE

VAL

GLN

ASN

GLY

LYS

6

HIS

PHE

LYS

PHE

THR

ILE

THR

ALA

GLY

SER

7

LYS

VAL

ILE

GLN

ASN

GLU

PHE

THR

VAL

GLY

8

GLU

CYS

GLU

LEU

GLU

THR

MET

THR

GLY

9

GLU

LYS

VAL

LYS

THR

VAL

GLN

LEU

GLU

10

GLY

ASP

ASN

LYS

LEU

VAL

THR

ALA

PHE

LYS

11

ASN

ILE

LYS

SER

VAL

THR

GLU

LEU

ASN

GLY

12

ASP

ILE

THR

ASN

THR

MET

THR

LEU

GLY

13

ASP

ILE

VAL

PHE

LYS

ARG

ILE

SER

LYS

ARG

14

ILE

LEU

VAL

PRO

ARG

Samples:

sample_1: human liver fatty acid binding protein, [U-100% 13C; U-100% 15N], 0.5 mM; TAUROCHOLIC ACID, natural abundant, 7.5 mM; sodium phosphate, natural abundant, 10 mM; H2O, natural abundant, 93%; D2O, [U-2H], 7%

sample_conditions_1: ionic strength: 10 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis

NMR spectrometers:

Bruker Avance 600 MHz

BMRB	17302 17303 19160 19188 19189
PDB	2F73 2L67 2L68 2LKK 2PY1 3B2H 3B2I 3B2J 3B2K 3B2L 3STK 3STM 3STN 3VG2 3VG3 3VG4 3VG5 3VG6 3VG7
DBJ	BAI46102
EMBL	CAG46887
GB	AAA52418 AAA52419 AAH22287 AAH32801 AAX37108
REF	NP_001434 XP_001140263 XP_003268834 XP_003805902 XP_004029642
SP	P07148
AlphaFold	P07148