BMRB Entry 25330

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25330

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Title:
Backbone Assignment of the heterodimer HUAB from E. coli at 293K
Deposition date:
2014-11-12
Original release date:
2015-05-19
Authors:
Le Meur, Remy; Castaing, Bertrand; Landon, Celine; Loth, Karine
Citation:

Citation: Le Meur, Remy; Castaing, Bertrand; Landon, Celine; Loth, Karine. "Backbone assignment of the three dimers of HU from Escherichia coli at 293 K: EcHUalpha2, EcHUbeta2 2 and EcHUalphabeta2"  Biomol. NMR Assignments 9, 359-363 (2015).
PubMed: 25924603

Assembly members:

Assembly members:
HUAB-A, polymer, 90 residues, Formula weight is not available
HUAB-B, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E.coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJES-hupA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HUAB-A: MNKTQLIDVIAEKAELSKTQ AKAALESTLAAITESLKEGD AVQLVGFGTFKVNHRAERTG RNPQTGKEIKIAAANVPAFV SGKALKDAVK
HUAB-B: MNKSQLIDKIAAGADISKAA AGRALDAIIASVTESLKEGD DVALVGFGTFAVKERAARTG RNPQTGKEITIAAAKVPSFR AGKALKDAVN

Data sets:
Data typeCount
13C chemical shifts501
15N chemical shifts168
1H chemical shifts168

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HUAB_11
2HUAB_22

Entities:

Entity 1, HUAB_1 90 residues - Formula weight is not available

1   METASNLYSTHRGLNLEUILEASPVALILE
2   ALAGLULYSALAGLULEUSERLYSTHRGLN
3   ALALYSALAALALEUGLUSERTHRLEUALA
4   ALAILETHRGLUSERLEULYSGLUGLYASP
5   ALAVALGLNLEUVALGLYPHEGLYTHRPHE
6   LYSVALASNHISARGALAGLUARGTHRGLY
7   ARGASNPROGLNTHRGLYLYSGLUILELYS
8   ILEALAALAALAASNVALPROALAPHEVAL
9   SERGLYLYSALALEULYSASPALAVALLYS

Entity 2, HUAB_2 90 residues - Formula weight is not available

1   METASNLYSSERGLNLEUILEASPLYSILE
2   ALAALAGLYALAASPILESERLYSALAALA
3   ALAGLYARGALALEUASPALAILEILEALA
4   SERVALTHRGLUSERLEULYSGLUGLYASP
5   ASPVALALALEUVALGLYPHEGLYTHRPHE
6   ALAVALLYSGLUARGALAALAARGTHRGLY
7   ARGASNPROGLNTHRGLYLYSGLUILETHR
8   ILEALAALAALALYSVALPROSERPHEARG
9   ALAGLYLYSALALEULYSASPALAVALASN

Samples:

sample_1: HUAB-A, [U-99% 13C; U-99% 15N], 0.5 mM; HUAB-B 0.75 mM; sodium chloride 200 mM; potassium phosphate 20 mM; DTT 0.05 mM; H2O 95%; D2O 5%

sample_2: HUAB-A 0.75 mM; HUAB-B, [U-99% 13C; U-99% 15N], 0.5 mM; sodium chloride 200 mM; potassium phosphate 20 mM; DTT 0.05 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.22 M; pH: 7; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks