BMRB Entry 25283

Title:
Solution structure of scoloptoxin SSD609 from Scolopendra mutilans
Deposition date:
2014-10-14
Original release date:
2015-09-04
Authors:
Wu, Fangming; Sun, Peibei; Wang, Chenyang; He, Yao; Zhang, Longhua; Tian, Changlin
Citation:

Citation: Sun, Peibei; Wu, Fangming; Wen, Ming; Yang, Xingwang; Wang, Chenyang; Li, Yiming; He, Shufang; Zhang, Longhua; Zhang, Yun; Tian, Changlin. "A distinct three-helix centipede toxin SSD609 inhibits Iks channels by interacting with the KCNE1 auxiliary subunit"  Sci. Rep. 5, 13399-13399 (2015).
PubMed: 26307551

Assembly members:

Assembly members:
SSD609, polymer, 47 residues, 5640.370 Da.

Natural source:

Natural source:   Common Name: Centipedes   Taxonomy ID: 251420   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Scolopendra mutilans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SSD609: ADDKCEDSLRREIACTKCRD RVRTDDYFYECCTSESTFKK CQTMLHQ

Data sets:
Data typeCount
1H chemical shifts243

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 47 residues - 5640.370 Da.

1   ALAASPASPLYSCYSGLUASPSERLEUARG
2   ARGGLUILEALACYSTHRLYSCYSARGASP
3   ARGVALARGTHRASPASPTYRPHETYRGLU
4   CYSCYSTHRSERGLUSERTHRPHELYSLYS
5   CYSGLNTHRMETLEUHISGLN

Samples:

sample_1: SSD609 1 mM; TRIS 50 mM; sodium chloride 100 mM; calcium chloride 5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.115 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Varian INOVA 700 MHz

Related Database Links:

PDB