BMRB Entry 25279

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25279

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Title:
Met e- and Ile d1-methyl 1H and 13C Chemical Shift Assignments for the HECT domain of the human Smurf2 ubiquitin ligase
Deposition date:
2014-10-09
Original release date:
2022-05-12
Authors:
Mari, Sara; Ruetalo, Natalia; Maspero, Elena; Stoffregen, Mira; Pasqualato, Sebastiano; Polo, Simona; Wiesner, Silke
Citation:

Citation: Mari, Sara; Ruetalo, Natalia; Maspero, Elena; Stoffregen, Mira; Pasqualato, Sebastiano; Polo, Simona; Wiesner, Silke. "Structural and functional framework for the autoinhibition of Nedd4-family ubiquitin ligases"  Structure 22, 1639-1649 (2014).
PubMed: 25438670

Assembly members:

Assembly members:
Smurf2, polymer, 387 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pProEx HTb

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Smurf2: GAMAPRYKRDLVQKLKILRQ ELSQQQPQAGHCRIEVSREE IFEESYRQVMKMRPKDLWKR LMIKFRGEEGLDYGGVAREW LYLLSHEMLNPYYGLFQYSR DDIYTLQINPDSAVNPEHLS YFHFVGRIMGMAVFHGHYID GGFTLPFYKQLLGKSITLDD MELVDPDLHNSLVWILENDI TGVLDHTFCVEHNAYGEIIQ HELKPNGKSIPVNEENKKEY VRLYVNWRFLRGIEAQFLAL QKGFNEVIPQHLLKTFDEKE LELIICGLGKIDVNDWKVNT RLKHCTPDSNIVKWFWKAVE FFDEERRARLLQFVTGSSRV PLQGFKALQGAAGPRLFTIH QIDACTNNLPKAHTCFNRID IPPYESYEKLYEKLLTAIEE TCGFAVE

Data sets:
Data typeCount
13C chemical shifts29
1H chemical shifts87

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 387 residues - Formula weight is not available

1   GLYALAMETALAPROARGTYRLYSARGASP
2   LEUVALGLNLYSLEULYSILELEUARGGLN
3   GLULEUSERGLNGLNGLNPROGLNALAGLY
4   HISCYSARGILEGLUVALSERARGGLUGLU
5   ILEPHEGLUGLUSERTYRARGGLNVALMET
6   LYSMETARGPROLYSASPLEUTRPLYSARG
7   LEUMETILELYSPHEARGGLYGLUGLUGLY
8   LEUASPTYRGLYGLYVALALAARGGLUTRP
9   LEUTYRLEULEUSERHISGLUMETLEUASN
10   PROTYRTYRGLYLEUPHEGLNTYRSERARG
11   ASPASPILETYRTHRLEUGLNILEASNPRO
12   ASPSERALAVALASNPROGLUHISLEUSER
13   TYRPHEHISPHEVALGLYARGILEMETGLY
14   METALAVALPHEHISGLYHISTYRILEASP
15   GLYGLYPHETHRLEUPROPHETYRLYSGLN
16   LEULEUGLYLYSSERILETHRLEUASPASP
17   METGLULEUVALASPPROASPLEUHISASN
18   SERLEUVALTRPILELEUGLUASNASPILE
19   THRGLYVALLEUASPHISTHRPHECYSVAL
20   GLUHISASNALATYRGLYGLUILEILEGLN
21   HISGLULEULYSPROASNGLYLYSSERILE
22   PROVALASNGLUGLUASNLYSLYSGLUTYR
23   VALARGLEUTYRVALASNTRPARGPHELEU
24   ARGGLYILEGLUALAGLNPHELEUALALEU
25   GLNLYSGLYPHEASNGLUVALILEPROGLN
26   HISLEULEULYSTHRPHEASPGLULYSGLU
27   LEUGLULEUILEILECYSGLYLEUGLYLYS
28   ILEASPVALASNASPTRPLYSVALASNTHR
29   ARGLEULYSHISCYSTHRPROASPSERASN
30   ILEVALLYSTRPPHETRPLYSALAVALGLU
31   PHEPHEASPGLUGLUARGARGALAARGLEU
32   LEUGLNPHEVALTHRGLYSERSERARGVAL
33   PROLEUGLNGLYPHELYSALALEUGLNGLY
34   ALAALAGLYPROARGLEUPHETHRILEHIS
35   GLNILEASPALACYSTHRASNASNLEUPRO
36   LYSALAHISTHRCYSPHEASNARGILEASP
37   ILEPROPROTYRGLUSERTYRGLULYSLEU
38   TYRGLULYSLEULEUTHRALAILEGLUGLU
39   THRCYSGLYPHEALAVALGLU

Samples:

sample_1: Smurf2 HECT domain, [U-1H,13C Met e- and Ile d1-methyl; 2H,12C], 40 uM; D2O 100%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HMQCsample_1isotropicsample_conditions_1

Software:

XEASY, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz