Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25268
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Tian, Fang; Rill, Richard; Castaining, Jean-Philippe; Hsin, Jen; Tan, Irene; Wang, Xingshen; Huang, Kerwyn-Casey; Ramamurthi, Kumaran. "Structural and mechanistic basis for the geometric-driven subcellular localication of a small protein" Nat. Struct. Biol. ., .-..
Assembly members:
Molecule_1, polymer, 26 residues, 3022.7605 Da.
Natural source: Common Name: firmicutes Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pKR205
Entity Sequences (FASTA):
Molecule_1: MKFYTIKLPKFLGGIVRAML
GSFRKD
Data type | Count |
1H chemical shifts | 172 |
13C chemical shifts | 89 |
15N chemical shifts | 22 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | entity | 1 |
Entity 1, entity 26 residues - 3022.7605 Da.
1 | MET | LYS | PHE | TYR | THR | ILE | LYS | LEU | PRO | LYS | ||||
2 | PHE | LEU | GLY | GLY | ILE | VAL | ARG | ALA | MET | LEU | ||||
3 | GLY | SER | PHE | ARG | LYS | ASP |
sample_1: SpoVM, [U-100% 13C; U-100% 15N], 0.5 1 mM; sodium phosphate 16 mM; sodium chloride 80 mM; H2O 95%; D2O 5%
sample_conditions_1: temperature: 310 K; pH: 6.5; pressure: 1 atm; ionic strength: 80 mM
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
NMRView, Johnson, One Moon Scientific - data analysis
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
PDB | |
EMBL | CAA74254 CAB13454 |
GB | AAC36809 AAU23337 AAU40697 ADM37675 ADV96607 |
REF | NP_389463 WP_003221545 |
SP | P37817 |
AlphaFold | P37817 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks