BMRB Entry 25264
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25264
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Clark, Sarah; Barbar, Elisar. "ctNsa-2, 1-84" The BMRB entry is the only known published source for the data..
Assembly members:
Nsa2, polymer, 98 residues, Formula weight is not available
Natural source: Common Name: ascomycetes Taxonomy ID: 209285 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Chaetomium thermophilum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24
Entity Sequences (FASTA):
Nsa2: DYDIPTTENLYFQGAHMPQN
EYIERHRKLHGRRLDAEERA
RKKAAREGHKNSENAQNLRG
LRAKLYAKQRHAQKIQMRKA
IKQHEERNVKGRPAEKEP
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 89 |
| 13C chemical shifts | 176 |
| 15N chemical shifts | 89 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 60S Pre-ribosome | 1 |
Entities:
Entity 1, 60S Pre-ribosome 98 residues - Formula weight is not available
| 1 | ASP | TYR | ASP | ILE | PRO | THR | THR | GLU | ASN | LEU | ||||
| 2 | TYR | PHE | GLN | GLY | ALA | HIS | MET | PRO | GLN | ASN | ||||
| 3 | GLU | TYR | ILE | GLU | ARG | HIS | ARG | LYS | LEU | HIS | ||||
| 4 | GLY | ARG | ARG | LEU | ASP | ALA | GLU | GLU | ARG | ALA | ||||
| 5 | ARG | LYS | LYS | ALA | ALA | ARG | GLU | GLY | HIS | LYS | ||||
| 6 | ASN | SER | GLU | ASN | ALA | GLN | ASN | LEU | ARG | GLY | ||||
| 7 | LEU | ARG | ALA | LYS | LEU | TYR | ALA | LYS | GLN | ARG | ||||
| 8 | HIS | ALA | GLN | LYS | ILE | GLN | MET | ARG | LYS | ALA | ||||
| 9 | ILE | LYS | GLN | HIS | GLU | GLU | ARG | ASN | VAL | LYS | ||||
| 10 | GLY | ARG | PRO | ALA | GLU | LYS | GLU | PRO |
Samples:
sample_1: Nsa2, [U-100% 13C; U-100% 15N], 0.3 mM; NaCl 5 mM; H2O 90%; D2O 90%
sample_conditions_1: temperature: 273 K; pH: 7.4; pressure: 1 atm; ionic strength: 5 mM
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe v5.5, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY v3.115, Goddard - chemical shift assignment
CS-Rosetta, Shen, Vernon, Baker and Bax - structure solution
NMRDraw v5.5, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks