BMRB Entry 25240

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25240
MolProbity Validation Chart

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NMR-STAR v3 text file.
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Title:
Solution structure of Twinstar from Drosophila melanogastor
Deposition date:
2014-09-20
Original release date:
2015-09-21
Authors:
Shukla, Vaibhav; Maheshwari, Diva; Kumar, Dinesh; Arora, Ashish
Citation:

Citation: Shukla, Vaibhav; Maheshwari, Diva; Jain, Anupam; Tripathi, Sarita; Kumar, Dinesh; Arora, Ashish. "Solution stucture and dynamics of Twinstar from Drosophila melanogastor"  .

Assembly members:

Assembly members:
entity, polymer, 148 residues, 17180.645 Da.

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETNH6

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MASGVTVSDVCKTTYEEIKK DKKHRYVIFYIRDEKQIDVE TVADRNAEYDQFLEDIQKCG PGECRYGLFDFEYMHQCQGT SESSKKQKLFLMSWCPDTAK VKKKMLYSSSFDALKKSLVG VQKYIQATDLSEASREAVEE KLRATDRQ

Data sets:
Data typeCount
13C chemical shifts652
15N chemical shifts146
1H chemical shifts1008

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 148 residues - 17180.645 Da.

1   METALASERGLYVALTHRVALSERASPVAL
2   CYSLYSTHRTHRTYRGLUGLUILELYSLYS
3   ASPLYSLYSHISARGTYRVALILEPHETYR
4   ILEARGASPGLULYSGLNILEASPVALGLU
5   THRVALALAASPARGASNALAGLUTYRASP
6   GLNPHELEUGLUASPILEGLNLYSCYSGLY
7   PROGLYGLUCYSARGTYRGLYLEUPHEASP
8   PHEGLUTYRMETHISGLNCYSGLNGLYTHR
9   SERGLUSERSERLYSLYSGLNLYSLEUPHE
10   LEUMETSERTRPCYSPROASPTHRALALYS
11   VALLYSLYSLYSMETLEUTYRSERSERSER
12   PHEASPALALEULYSLYSSERLEUVALGLY
13   VALGLNLYSTYRILEGLNALATHRASPLEU
14   SERGLUALASERARGGLUALAVALGLUGLU
15   LYSLEUARGALATHRASPARGGLN

Samples:

sample_1: ammonium sulfate, [U-99% 15N], 0.1 % w/v; sodium phosphate 20 mM; sodium chloride 50 mM; DTT 1 mM; sodium azide 0.1 % w/v

sample_2: ammonium sulfate, [U-99% 15N], 0.1 % w/v; Glucose, [U-100% 13C], 0.2 % w/v; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1 % w/v; DTT 1 mM

sample_3: ammonium sulfate, [U-99% 15N], 0.1 % w/v; Glucose, [U-100% 13C], 0.2 % w/v; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1 % w/v; DTT 1 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
HNCANHsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_3isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_3isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
CBHDsample_3isotropicsample_conditions_1
CBHEsample_3isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

TOPSPIN, Bruker Biospin - processing

PSVS, Bhattacharya and Montelione - structure Validation

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAA19856 AAC46962 AAC46963 AAF47146 AAR09835
REF NP_477034 XP_001360888 XP_001648106 XP_001648107 XP_001648108
SP P45594
AlphaFold P45594

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks