BMRB Entry 25231

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25231

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Title:
14-3-3 Zeta Backbone Assignment
Deposition date:
2014-09-16
Original release date:
2015-04-28
Authors:
Killoran, Ryan; Fan, Jingsong; Yang, Daiwen; Choy, Wing-Yiu
Citation:

Citation: Killoran, Ryan; Fan, Jingsong; Yang, Daiwen; Shilton, Brian; Choy, Wing-Yiu. "Structural Analysis of the 14-3-3 zeta /Chibby Interaction Involved in Wnt/ beta-Catenin Signaling"  Plos One 10, e0123934-e0123934 (2015).
PubMed: 25909186

Assembly members:

Assembly members:
14-3-3, polymer, 234 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E.coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pDEST17

Entity Sequences (FASTA):

Entity Sequences (FASTA):
14-3-3: GMDKNELVQKAKLAEQAERY DDMAACMKSVTEQGAELSNE ERNLLSVAYKNVVGARRSSW RVVSSIEQKTEGAEKKQQMA REYREKIETELRDICNDVLS LLEKFLIPNASQAESKVFYL KMKGDYYRYLAEVAAGDDKK GIVDQSQQAYQEAFEISKKE MQPTHPIRLGLALNFSVFYY EILNSPEKACSLAKTAFDEA IAELDTLSEESYKDSTLIMQ LLRDNLTLWTSDTQ

Data sets:
Data typeCount
13C chemical shifts377
15N chemical shifts189
1H chemical shifts190

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 234 residues - Formula weight is not available

1   GLYMETASPLYSASNGLULEUVALGLNLYS
2   ALALYSLEUALAGLUGLNALAGLUARGTYR
3   ASPASPMETALAALACYSMETLYSSERVAL
4   THRGLUGLNGLYALAGLULEUSERASNGLU
5   GLUARGASNLEULEUSERVALALATYRLYS
6   ASNVALVALGLYALAARGARGSERSERTRP
7   ARGVALVALSERSERILEGLUGLNLYSTHR
8   GLUGLYALAGLULYSLYSGLNGLNMETALA
9   ARGGLUTYRARGGLULYSILEGLUTHRGLU
10   LEUARGASPILECYSASNASPVALLEUSER
11   LEULEUGLULYSPHELEUILEPROASNALA
12   SERGLNALAGLUSERLYSVALPHETYRLEU
13   LYSMETLYSGLYASPTYRTYRARGTYRLEU
14   ALAGLUVALALAALAGLYASPASPLYSLYS
15   GLYILEVALASPGLNSERGLNGLNALATYR
16   GLNGLUALAPHEGLUILESERLYSLYSGLU
17   METGLNPROTHRHISPROILEARGLEUGLY
18   LEUALALEUASNPHESERVALPHETYRTYR
19   GLUILELEUASNSERPROGLULYSALACYS
20   SERLEUALALYSTHRALAPHEASPGLUALA
21   ILEALAGLULEUASPTHRLEUSERGLUGLU
22   SERTYRLYSASPSERTHRLEUILEMETGLN
23   LEULEUARGASPASNLEUTHRLEUTRPTHR
24   SERASPTHRGLN

Samples:

14-3-3: 14-3-3, [U-100% 13C; U-100% 15N; U-80% 2H], 0.5 mM; DSS 0.1 mM; sodium chloride 100 mM; sodium phosphate 50 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.2 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC14-3-3isotropicsample_conditions_1
3D HNCACB14-3-3isotropicsample_conditions_1
3D CBCA(CO)NH14-3-3isotropicsample_conditions_1
3D 1H-15N NOESY14-3-3isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAA04534 BAA06402 BAA11464 BAA11751 BAA13421
EMBL CAG31814 CAH92765
GB AAA30514 AAA36446 AAA80544 AAB22282 AAB22943
PRF 2022313B
REF NP_001026514 NP_001126612 NP_001129171 NP_001129172 NP_001129173
SP P29361 P63101 P63102 P63103 P63104
TPG DAA18059 DAA22580
AlphaFold P29361 P63101 P63102 P63103 P63104

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks