BMRB Entry 25214

Name: HIGH-RESOLUTION NMR STRUCTURES OF THE DOMAINS OF SACCHAROMYCES CEREVISIAE THO1
Published: 2014-12-15

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PDB ID: 4uzx
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25214
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

HIGH-RESOLUTION NMR STRUCTURES OF THE DOMAINS OF SACCHAROMYCES CEREVISIAE THO1

Deposition date:

2014-09-10

Original release date:

2014-12-15

Authors:

Jacobsen, J.; Allen, Mark; Freund, S.; Bycroft, M.

Citation:

Citation: Jacobsen, J.; Allen, Mark; Freund, S.; Bycroft, M.. "HIGH-RESOLUTION NMR STRUCTURES OF THE DOMAINS OF SACCHAROMYCES CEREVISIAE THO1" .

Assembly members:

Assembly members:
PROTEIN_THO1, polymer, 67 residues, 7490.4826 Da.

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: na

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PROTEIN_THO1: GSALSPEEIKAKALDLLNKK LHRANKFGQDQADIDSLQRQ INRVEKFGVDLNSKLAEELG LVSRKNE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
1H chemical shifts	496
13C chemical shifts	225
15N chemical shifts	66

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PROTEIN THO1	1

Entities:

Entity 1, PROTEIN THO1 67 residues - 7490.4826 Da.

1

GLY

SER

ALA

LEU

SER

PRO

GLU

ILE

LYS

2

ALA

LYS

ALA

LEU

ASP

LEU

ASN

LYS

3

LEU

HIS

ARG

ALA

ASN

LYS

PHE

GLY

GLN

ASP

4

GLN

ALA

ASP

ILE

ASP

SER

LEU

GLN

ARG

GLN

5

ILE

ASN

ARG

VAL

GLU

LYS

PHE

GLY

VAL

ASP

6

LEU

ASN

SER

LYS

LEU

ALA

GLU

LEU

GLY

7

LEU

VAL

SER

ARG

LYS

ASN

GLU

Samples:

sample_1: PROTEIN THO1, [U-13C; U-15N], 1.5 mM; potassium phosphate 20 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 120.000 mM; pH: 6.500; pressure: 1.000 atm; temperature: 293.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	sample_1	solution	sample_conditions_1
1H-13C HSQC	sample_1	solution	sample_conditions_1
HNCACB	sample_1	solution	sample_conditions_1
CBCA(CO)NH	sample_1	solution	sample_conditions_1
HNCO	sample_1	solution	sample_conditions_1
HN(CA)CO	sample_1	solution	sample_conditions_1
HNHB	sample_1	solution	sample_conditions_1
1H-NOESY	sample_1	solution	sample_conditions_1
1H-DQF-COSY	sample_1	solution	sample_conditions_1
1H-TOCSY	sample_1	solution	sample_conditions_1

Software:

Ansig vany, Kraulis - chemical shift assignment

AutoDep v4.3, PDBe - chemical shift assignment

CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment, refinement

NMR spectrometers:

Bruker Avance 500 MHz
Bruker Avance 600 MHz
Bruker Avance 800 MHz

UNP	THO1_YEAST
PDB	4UZX
DBJ	GAA22895
EMBL	CAY79235
GB	AAB64599 AAS56105 AHY75622 AJU40024 AJU40279
REF	NP_010985
SP	P40040
TPG	DAA07722
AlphaFold	D3DLW8 P40040