BMRB Entry 25213

Title:
HIGH-RESOLUTION NMR STRUCTURES OF THE DOMAINS OF SACCHAROMYCES CEREVISIAE THO1
Deposition date:
2014-09-10
Original release date:
2014-12-15
Authors:
Jacobsen, J.; Allen, Mark; Freund, S.; Bycroft, M.
Citation:

Citation: Jacobsen, J.; Allen, Mark; Freund, S.; Bycroft, M.. "HIGH-RESOLUTION NMR STRUCTURES OF THE DOMAINS OF SACCHAROMYCES CEREVISIAE THO1"  .

Assembly members:

Assembly members:
PROTEIN_THO1, polymer, 50 residues, 5516.143 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: na

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PROTEIN_THO1: SADYSSLTVVQLKDLLTKRN LSVGGLKNELVQRLIKDDEE SKGESEVSPQ

Data sets:
Data typeCount
1H chemical shifts366
13C chemical shifts175
15N chemical shifts48

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PROTEIN THO11

Entities:

Entity 1, PROTEIN THO1 50 residues - 5516.143 Da.

1   SERALAASPTYRSERSERLEUTHRVALVAL
2   GLNLEULYSASPLEULEUTHRLYSARGASN
3   LEUSERVALGLYGLYLEULYSASNGLULEU
4   VALGLNARGLEUILELYSASPASPGLUGLU
5   SERLYSGLYGLUSERGLUVALSERPROGLN

Samples:

sample_1: PROTEIN THO1, [U-13C; U-15N], 1.5 mM; potassium phosphate 20 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 120.000 mM; pH: 6.500; pressure: 1.000 atm; temperature: 293.000 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1solutionsample_conditions_1
1H-13C HSQCsample_1solutionsample_conditions_1
HNCACBsample_1solutionsample_conditions_1
CBCA(CO)NHsample_1solutionsample_conditions_1
HNCOsample_1solutionsample_conditions_1
HN(CA)COsample_1solutionsample_conditions_1
HNHBsample_1solutionsample_conditions_1
1H-NOESYsample_1solutionsample_conditions_1
1H-DQF-COSYsample_1solutionsample_conditions_1
1H-TOCSYsample_1solutionsample_conditions_1

Software:

Ansig vany, Kraulis - chemical shift assignment

AutoDep v4.3, PDBe - chemical shift assignment

CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment, refinement

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

UNP THO1_YEAST
PDB
EMBL CAY79235
GB AAB64599 AAS56105 AHY75622 AJU40024 AJU40279
REF NP_010985
SP P40040
TPG DAA07722
AlphaFold D3DLW8 P40040

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks