BMRB Entry 25185

Name: Backbone chemical shift assignment of FG-NUP (48-172) in buffer condition.
Published: 2015-10-08

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25185

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone chemical shift assignment of FG-NUP (48-172) in buffer condition.

Deposition date:

2014-08-29

Original release date:

2015-10-08

Authors:

Dutta, Kaushik; Cowburn, David; Rout, Michael

Citation:

Citation: Hough, Loren; Dutta, Kaushik; Sparks, Sam; Temel, Deniz; Kamal, Alia; Tetenbaum-Novatt, Jaclyn; Rout, Michael; Cowburn, David. "Atomic Scale Dynamic Behavior of the Nuclear Transport Selectivity Barrier" Science ., .-..

Assembly members:

Assembly members:
FG-N-6His, polymer, 137 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FG-N-6His: MGTSAPNNTNNANSSITPAF GSNNTGNTAFGNSNPTSNVF GSNNSTTNTFGSNSAGTSLF GSSSAQQTKSNGTAGGNTFG SSSLFNNSTNSNTTKPAFGG LNFGGGNNTTPSSTGNANTS NNLFGATASHMHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	352
15N chemical shifts	120
1H chemical shifts	120

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity	1

Entities:

Entity 1, entity 137 residues - Formula weight is not available

1

MET

GLY

THR

SER

ALA

PRO

ASN

THR

ASN

2

ASN

ALA

ASN

SER

ILE

THR

PRO

ALA

PHE

3

GLY

SER

ASN

THR

GLY

ASN

THR

ALA

PHE

4

GLY

ASN

SER

ASN

PRO

THR

SER

ASN

VAL

PHE

5

GLY

SER

ASN

SER

THR

ASN

THR

PHE

6

GLY

SER

ASN

SER

ALA

GLY

THR

SER

LEU

PHE

7

GLY

SER

ALA

GLN

THR

LYS

SER

8

ASN

GLY

THR

ALA

GLY

ASN

THR

PHE

GLY

9

SER

LEU

PHE

ASN

SER

THR

ASN

10

SER

ASN

THR

LYS

PRO

ALA

PHE

GLY

11

LEU

ASN

PHE

GLY

ASN

THR

12

PRO

SER

THR

GLY

ASN

ALA

ASN

THR

SER

13

ASN

LEU

PHE

GLY

ALA

THR

ALA

SER

HIS

14

MET

HIS

Samples:

sample_1: FG-N-6His, [U-100% 15N], 250 uM; HEPES 20 mM; KCl 150 mM; MgCl2 2 mM; BSA 100 mM; H2O 90%; D2O 10%

sample_2: FG-N-6His, [U-100% 13C; U-100% 15N], 250 uM; HEPES 20 mM; KCl 150 mM; MgCl2 2 mM; BSA 100 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HN(CO)CA	sample_2	isotropic	sample_conditions_1
3D HN(CA)CO	sample_2	isotropic	sample_conditions_1
3D C(CO)NH	sample_2	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 800 MHz
Bruker Avance 900 MHz

BMRB	25184
DBJ	GAA24320
EMBL	CAA89332 CAY80739
GB	AAA34821 AHY78965 AJP39666 AJR53935 AJR54261
REF	NP_012494
SP	P14907
TPG	DAA08759
AlphaFold	P14907