BMRB Entry 25183

Name: Backbone chemical shift assignment of FG-NUP (274 -398) in buffer condition.
Published: 2015-10-08

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25183

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone chemical shift assignment of FG-NUP (274 -398) in buffer condition.

Deposition date:

2014-08-29

Original release date:

2015-10-08

Authors:

Dutta, Kaushik; Cowburn, David; Rout, Michael

Citation:

Citation: Hough, Loren; Dutta, Kaushik; Sparks, Sam; Temel, Deniz; Kamal, Alia; Tetenbaum-Novatt, Jaclyn; Rout, Michael; Cowburn, David. "Atomic Scale Dynamic Behavior of the Nuclear Transport Selectivity Barrier" Science ., .-..

Assembly members:

Assembly members:
FSFG-K-6His, polymer, 133 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FSFG-K-6His: MDNKTTNTTPSFSFGAKSDE NKAGATSKPAFSFGAKPEEK KDDNSSKPAFSFGAKSNEDK QDGTAKPAFSFGAKPAEKNN NETSKPAFSFGAKSDEKKDG DASKPAFSFGAKPDENKASA TSKPALEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	361
15N chemical shifts	113
1H chemical shifts	113

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity	1

Entities:

Entity 1, entity 133 residues - Formula weight is not available

1

MET

ASP

ASN

LYS

THR

ASN

THR

PRO

2

SER

PHE

SER

PHE

GLY

ALA

LYS

SER

ASP

GLU

3

ASN

LYS

ALA

GLY

ALA

THR

SER

LYS

PRO

ALA

4

PHE

SER

PHE

GLY

ALA

LYS

PRO

GLU

LYS

5

LYS

ASP

ASN

SER

LYS

PRO

ALA

PHE

6

SER

PHE

GLY

ALA

LYS

SER

ASN

GLU

ASP

LYS

7

GLN

ASP

GLY

THR

ALA

LYS

PRO

ALA

PHE

SER

8

PHE

GLY

ALA

LYS

PRO

ALA

GLU

LYS

ASN

9

ASN

GLU

THR

SER

LYS

PRO

ALA

PHE

SER

PHE

10

GLY

ALA

LYS

SER

ASP

GLU

LYS

ASP

GLY

11

ASP

ALA

SER

LYS

PRO

ALA

PHE

SER

PHE

GLY

12

ALA

LYS

PRO

ASP

GLU

ASN

LYS

ALA

SER

ALA

13

THR

SER

LYS

PRO

ALA

LEU

GLU

HIS

14

HIS

Samples:

sample_1: FSFG-K-6His, [U-100% 15N], 250 uM; H2O 90%; D2O 10%; HEPES 20 mM; KCL 150 mM; MgCl2 2 mM

sample_2: FSFG-K-6His, [U-100% 13C; U-100% 15N], 250 uM; H2O 90%; D2O 10%; HEPES 20 mM; KCL 150 mM; MgCl2 2 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HN(CO)CA	sample_2	isotropic	sample_conditions_1
3D HN(CA)CO	sample_2	isotropic	sample_conditions_1
3D C(CO)NH	sample_2	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 800 MHz
Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks