BMRB Entry 25166

Name: 1H, 15N, and 13C resonance assignments of the ectodomain of the B. subtilis RodZ
Published: 2015-10-23

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25166

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N, and 13C resonance assignments of the ectodomain of the B. subtilis RodZ

Deposition date:

2014-08-20

Original release date:

2015-10-23

Authors:

Pereira, Ana; Saraiva, Ivo; Matzapetakis, Manolis; Henriques, Adriano; Costa, Teresa; Paiva, Ana

Citation:

Citation: Pereira, Ana; Paiva, Ana; Saraiva, Ivo; Henriques, Adriano; Matzapetakis, Manolis. "1H, 15N, and 13C resonance assignments of the ectodomain of the B. subtilis RodZ" Biomol. NMR Assign. 9, 285-288 (2015).
PubMed: 25503291

Assembly members:

Assembly members:
RodZC, polymer, 127 residues, 14257.9732 Da.

Natural source:

Natural source: Common Name: B. subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTC248

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RodZC: MGHHHHHHSSGENDSEKLEI KAAGTEGSLTTYEVSGADKI ELELKASDSSWIRVRDENSS SLKEGTLKKDETYKKDITDQ KQVDIRTGYAPNLKIKINGK VLSYELDPKKVMAQTIKIVN KKEEKSS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	480
15N chemical shifts	110
1H chemical shifts	777

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RodZC	1

Entities:

Entity 1, RodZC 127 residues - 14257.9732 Da.

1

MET

GLY

HIS

SER

2

GLY

GLU

ASN

ASP

SER

GLU

LYS

LEU

GLU

ILE

3

LYS

ALA

GLY

THR

GLU

GLY

SER

LEU

THR

4

THR

TYR

GLU

VAL

SER

GLY

ALA

ASP

LYS

ILE

5

GLU

LEU

GLU

LEU

LYS

ALA

SER

ASP

SER

6

TRP

ILE

ARG

VAL

ARG

ASP

GLU

ASN

SER

7

SER

LEU

LYS

GLU

GLY

THR

LEU

LYS

ASP

8

GLU

THR

TYR

LYS

ASP

ILE

THR

ASP

GLN

9

LYS

GLN

VAL

ASP

ILE

ARG

THR

GLY

TYR

ALA

10

PRO

ASN

LEU

LYS

ILE

LYS

ILE

ASN

GLY

LYS

11

VAL

LEU

SER

TYR

GLU

LEU

ASP

PRO

LYS

12

VAL

MET

ALA

GLN

THR

ILE

LYS

ILE

VAL

ASN

13

LYS

GLU

LYS

SER

Samples:

H2O: RodZC, [U-13C; U-15N], 1.0 mM; Sodium Chloride 100.0 mM; Sodium Azide 1.0 mM; Sodium Phospate 20.0 mM; H2O 90%; D2O 10%

D2O: RodZC, [U-13C; U-15N], 1.0 mM; Sodium Chloride 100.0 mM; Sodium Azide 1.0 mM; Sodium Phosphate 20.0 mM; H2O 10%; D2O 90%

standard: ionic strength: 0.210 M; pH: 6.800; pressure: 1.000 atm; temperature: 298.150 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC/HMQC	H2O	isotropic	standard
2D 1H-13C HSQC/HMQC	H2O	isotropic	standard
hCCH (hC_CH.relayed)	H2O	isotropic	standard
3D HNCO	H2O	isotropic	standard
3D HNCA	H2O	isotropic	standard
3D HNCACB	H2O	isotropic	standard
3D HN(CO)CA	H2O	isotropic	standard
2D 1H-1H TOCSY	D2O	isotropic	standard
2D 1H-13C HSQC/HMQC	H2O	isotropic	standard
2D 1H-1H COSY	D2O	isotropic	standard

Software:

CARA v2.1.5, K Wuthich - chemical shift assignment

CcpNmr_Analysis v2.4, CCPN - chemical shift assignment

TOPSPIN v2.1, Bruker - collection, processing

NMR spectrometers:

Bruker Avance 800 MHz

DBJ	BAI85320 BAM52337 BAM57913 GAK80670
EMBL	CAB13564 CCU58263 CEI56872 CEJ77279 CJS25636
GB	AAB47706 ADV96716 AEP90843 AFI28374 AFQ57624
REF	NP_389573 WP_003244699 WP_009967274 WP_009968950 WP_010327981
SP	O31771
AlphaFold	O31771