BMRB Entry 25161
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR25161
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Citation: Lohans, Christopher; Li, Jessica; Vederas, John. "Structure and Biosynthesis of Carnolysin, a Homolog of Enterococcal Cytolysin with D-Amino Acids" J. Am. Chem. Soc. 136, 13150-13153 (2014).
PubMed: 25207953
Assembly members:
CrnA1', polymer, 45 residues, Formula weight is not available
Natural source: Common Name: firmicutes Taxonomy ID: 2751 Superkingdom: Bacteria Kingdom: not available Genus/species: Carnobacterium maltaromaticum
Experimental source: Production method: purified from the natural source Host organism: Carnobacterium maltaromaticum Vector: N/A
Entity Sequences (FASTA):
CrnA1': GDINGEFXXXPACVYXVMVV
XKAAXAKCAAGAXAVXGAIL
XAIRC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 225 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Carnolysin A1' | 1 |
Entities:
Entity 1, Carnolysin A1' 45 residues - Formula weight is not available
| 1 | GLY | ASP | ILE | ASN | GLY | GLU | PHE | DBU | ABA | DHA | ||||
| 2 | PRO | ALA | CYS | VAL | TYR | DAL | VAL | MET | VAL | VAL | ||||
| 3 | DAL | LYS | ALA | ALA | DHA | ALA | LYS | CYS | ALA | ALA | ||||
| 4 | GLY | ALA | DAL | ALA | VAL | DAL | GLY | ALA | ILE | LEU | ||||
| 5 | DAL | ALA | ILE | ARG | CYS |
Samples:
sample_1: CrnA1' 1 mM; DSS 0.01%; H2O 90%; D2O 10%
sample_conditions_1: temperature: 273 K; pH: 6; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ, Varian - collection
NMR spectrometers:
- Varian VXRS 700 MHz