BMRB Entry 25154

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25154

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Title:
Backbone 1H, 15N and 13C resonance assignments of murine STING in complex with DMXAA
Deposition date:
2014-08-15
Original release date:
2015-03-23
Authors:
Lou, Yuan-Chao; Kao, Yi-Fen; Chin, Ko-Hsin; Chen, Jen-Kang; Tu, Je-Le; Chen, Chinpan; Chou, Shan-Ho
Citation:

Citation: Lou, Yuan-Chao; Kao, Yi-Fen; Chin, Ko-Hsin; Chen, Jen-Kang; Tu, Je-Le; Chen, Chinpan; Chou, Shan-Ho. "Backbone resonance assignments of the 54 kDa dimeric C-terminal domain of murine STING in complex with DMXAA"  Biomol. NMR Assign. 9, 271-274 (2015).
PubMed: 25487675

Assembly members:

Assembly members:
stimulators_of_interferon_genes_protein, polymer, 244 residues, Formula weight is not available
entity_1YE, non-polymer, 282.291 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
stimulators_of_interferon_genes_protein: SNALTPAEVSAVCEEKKLNV AHGLAWSYYIGYLRLILPGL QARIRMFNQLHNNMLSGAGS RRLYILFPLDCGVPDNLSVV DPNIRFRDMLPQQNIDRAGI KNRVYSNSVYEILENGQPAG VCILEYATPLQTLFAMSQDA KAGFSREDRLEQAKLFCRTL EEILEDVPESRNNCRLIVYQ EPTDGNSFSLSQEVLRHIRQ EEKEEVTMNAPMTSVAPPPS VLSQEPRLLISGMDQPLPLR TDLI

Data sets:
Data typeCount
13C chemical shifts663
15N chemical shifts221
1H chemical shifts234

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1stimulators_of_interferon_genes_protein, 11
2stimulators_of_interferon_genes_protein, 21
3DMXAA, 12
4DMXAA, 22

Entities:

Entity 1, stimulators_of_interferon_genes_protein, 1 244 residues - Formula weight is not available

Residues 1 to 3 are non-native sequence after TEV protease digestion. The 4th residue L is L138 in the native mouse STING protein. And this construct codes for mSTING138-378.

1   SERASNALALEUTHRPROALAGLUVALSER
2   ALAVALCYSGLUGLULYSLYSLEUASNVAL
3   ALAHISGLYLEUALATRPSERTYRTYRILE
4   GLYTYRLEUARGLEUILELEUPROGLYLEU
5   GLNALAARGILEARGMETPHEASNGLNLEU
6   HISASNASNMETLEUSERGLYALAGLYSER
7   ARGARGLEUTYRILELEUPHEPROLEUASP
8   CYSGLYVALPROASPASNLEUSERVALVAL
9   ASPPROASNILEARGPHEARGASPMETLEU
10   PROGLNGLNASNILEASPARGALAGLYILE
11   LYSASNARGVALTYRSERASNSERVALTYR
12   GLUILELEUGLUASNGLYGLNPROALAGLY
13   VALCYSILELEUGLUTYRALATHRPROLEU
14   GLNTHRLEUPHEALAMETSERGLNASPALA
15   LYSALAGLYPHESERARGGLUASPARGLEU
16   GLUGLNALALYSLEUPHECYSARGTHRLEU
17   GLUGLUILELEUGLUASPVALPROGLUSER
18   ARGASNASNCYSARGLEUILEVALTYRGLN
19   GLUPROTHRASPGLYASNSERPHESERLEU
20   SERGLNGLUVALLEUARGHISILEARGGLN
21   GLUGLULYSGLUGLUVALTHRMETASNALA
22   PROMETTHRSERVALALAPROPROPROSER
23   VALLEUSERGLNGLUPROARGLEULEUILE
24   SERGLYMETASPGLNPROLEUPROLEUARG
25   THRASPLEUILE

Entity 2, DMXAA, 1 - C17 H14 O4 - 282.291 Da.

1   1YE

Samples:

sample_1: MES 20 mM; sodium chloride 100 mM; sodium azide 1 mM; TCEP 10 mM; stimulators of interferon genes protein, [U-100% 13C; U-100% 15N; U-80% 2H], 1.25 mM; DMXAA 3.5 mM

sample_conditions_1: ionic strength: 200 mM; pH: 7; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.1, Bruker Biospin - collection

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks