BMRB Entry 25148

Name: NTD-NUSA
Published: 2015-02-16

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PDB ID: 2mt4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25148
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NTD-NUSA

Deposition date:

2014-08-12

Original release date:

2015-02-16

Authors:

Mobli, Mehdi; Lewis, Peter

Citation:

Citation: Mobli, Mehdi; Ma, Cong; Yang, Xiao; Keller, Andrew; King, Glenn; Lewis, Peter. "The Mechanism Of Transcription Pausing Enhancement By NusA" .

Assembly members:

Assembly members:
NTD-NUSA, polymer, 124 residues, 14019.991 Da.

Natural source:

Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETMCSIII

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NTD-NUSA: MSSELLDALTILEKEKGISK EIIIEAIEAALISAYKRNFN QAQNVRVDLNRETGSIRVFA RKDVVDEVYDQRLEISIEEA QGIHPEYMVGDVVEIEVTPK DFGRIAAQTAKQVVTQRVRE AERG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	878
13C chemical shifts	530
15N chemical shifts	121

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NTD-NUSA	1

Entities:

Entity 1, NTD-NUSA 124 residues - 14019.991 Da.

1

MET

SER

GLU

LEU

ASP

ALA

LEU

THR

2

ILE

LEU

GLU

LYS

GLU

LYS

GLY

ILE

SER

LYS

3

GLU

ILE

GLU

ALA

ILE

GLU

ALA

4

LEU

ILE

SER

ALA

TYR

LYS

ARG

ASN

PHE

ASN

5

GLN

ALA

GLN

ASN

VAL

ARG

VAL

ASP

LEU

ASN

6

ARG

GLU

THR

GLY

SER

ILE

ARG

VAL

PHE

ALA

7

ARG

LYS

ASP

VAL

ASP

GLU

VAL

TYR

ASP

8

GLN

ARG

LEU

GLU

ILE

SER

ILE

GLU

ALA

9

GLN

GLY

ILE

HIS

PRO

GLU

TYR

MET

VAL

GLY

10

ASP

VAL

GLU

ILE

GLU

VAL

THR

PRO

LYS

11

ASP

PHE

GLY

ARG

ILE

ALA

GLN

THR

ALA

12

LYS

GLN

VAL

THR

GLN

ARG

VAL

ARG

GLU

13

ALA

GLU

ARG

GLY

Samples:

sample_1: NTD-NUSA, [U-99% 13C; U-99% 15N], 400 uM; HEPES 10 mM; sodium chloride 150 mM

sample_conditions_1: temperature: 298 K; pH: 7.8; pressure: 1 atm; ionic strength: 0.16 M

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

TOPSPIN v3.1, Bruker Biospin - collection

Rowland_NMR_Toolkit v3, J. C. Hoch & A. S. Stern - processing

TALOS v+, Cornilescu, Delaglio and Bax - data analysis

CCPNMR v2.2, CCPN - peak picking

NMR spectrometers:

Bruker Avance 900 MHz

PDB	2MT4
DBJ	BAI85290 BAM52307 BAM57883 GAK80641
EMBL	CAA79231 CAB13533 CCU58233 CEI56842 CEJ77249
GB	ADM37756 ADV96687 AEP86650 AEP90813 AFI28344
REF	NP_389542 WP_003220941 WP_003231912 WP_010328008 WP_010334282
SP	P32727
AlphaFold	P32727