BMRB Entry 25147

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25147
MolProbity Validation Chart

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Title:
Struture of -24 DNA binding domain of sigma 54 from E. coli
Deposition date:
2014-08-12
Original release date:
2015-08-24
Authors:
Wemmer, David; Gao, Zhijuan; Pelton, Jeffrey
Citation:

Citation: Gao, Zhijuan; Pelton, Jeffrey; Wemmer, David. "Struture of -24 DNA binding domain of sigma 54 from E. coli"  .

Assembly members:

Assembly members:
entity, polymer, 84 residues, 9160.573 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: EASSTAIRALVKKLIAAENP AKPLSDSKLTSLLSEQGIMV ARRTVAKYRESLSIPPSNQR KQLVANSSSVDKLAAALEHH HHHH

Data sets:
Data typeCount
13C chemical shifts281
15N chemical shifts71
1H chemical shifts502

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 84 residues - 9160.573 Da.

1   GLUALASERSERTHRALAILEARGALALEU
2   VALLYSLYSLEUILEALAALAGLUASNPRO
3   ALALYSPROLEUSERASPSERLYSLEUTHR
4   SERLEULEUSERGLUGLNGLYILEMETVAL
5   ALAARGARGTHRVALALALYSTYRARGGLU
6   SERLEUSERILEPROPROSERASNGLNARG
7   LYSGLNLEUVALALAASNSERSERSERVAL
8   ASPLYSLEUALAALAALALEUGLUHISHIS
9   HISHISHISHIS

Samples:

sample_1: entity mM; sodium phosphate 20 mM; sodium chloride 200 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ BAA02315 BAB37504 BAE73474 BAE77246 BAG79010
EMBL CAA81617 CAD07837 CAP77662 CAQ33535 CAR00164
GB AAA27224 AAA58004 AAA62790 AAB60163 AAC76234
PIR AG0905
REF NP_312108 NP_417669 NP_457699 NP_462230 NP_709001
SP P24255 P26979
AlphaFold P24255 P26979

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks