BMRB Entry 25141

Title:
Backbone chemical shift assignment of EL_LovR bound to magnesium chloride
Deposition date:
2014-08-11
Original release date:
2016-06-30
Authors:
Ocasio, Victor; Correa, Fernando; Gardner, Kevin
Citation:

Citation: Ocasio, Victor; Correa, Fernando; Gardner, Kevin. "Ligand-induced folding of a two-component signaling receiver domain"  Biochemistry 54, 1353-1363 (2015).
PubMed: 25629646

Assembly members:

Assembly members:
EL_LovR, polymer, 125 residues, Formula weight is not available
MAGNESIUM ION, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: a-proteobacteria   Taxonomy ID: 39960   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Erythrobacter litoralis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHis-parallel

Data sets:
Data typeCount
13C chemical shifts232
15N chemical shifts70
1H chemical shifts70

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11
2MAGNESIUM ION2

Entities:

Entity 1, subunit 1 125 residues - Formula weight is not available

1   GLYALAMETGLYMETPROLYSVALLEUVAL
2   LEUGLUASPGLUPROLEUILEALAMETASN
3   LEUGLNTYRALAPHEGLUASPGLUGLYALA
4   GLUVALVALVALALAALATHRCYSGLUGLN
5   ALALEULYSSERLEUALAASPASNPROILE
6   ASPVALALAVALLEUASPVALASNLEUGLY
7   PROLYSSERHISCYSGLYPROVALALAASP
8   ALALEULYSGLNARGALAILEPROPHEILE
9   LEUHISTHRGLYASPLEUASPARGHISGLY
10   GLULEULEUARGLYSILEASPALAPROVAL
11   METALALYSPROALAASPTHRSERASPVAL
12   ALALYSARGALALEUGLUMETCYSGLYGLY
13   ASPLYSGLUPROALA

Entity 2, MAGNESIUM ION - Mg - 24.305 Da.

1   MG

Samples:

sample_1: magnesium chloride 10 mM; EL_LovR, [U-100% 13C; U-100% 15N], 500 uM; DTT 1 mM; sodium azide 3 mM; HEPES 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.025 M; pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks