BMRB Entry 25128
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25128
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Akcan, Muharrem; Clark, Richard; Daly, Norelle; Conibear, Anne; de Faoite, Andrew; Heghinian, Mari; Adams, David; Mari, Frank; Craik, David. "Transforming conotoxins into cyclotides: backbone cyclization of P-superfamily conotoxins" Biopolymers ., .-. (2015).
PubMed: 26172377
Assembly members:
cGm9a, polymer, 30 residues, 3075.419 Da.
Natural source: Common Name: Glory of the Sea Cone Taxonomy ID: 37336 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Conus gloriamaris
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
cGm9a: SCNNSCQSHSDCASHCICTF
RGCGAVNGLP
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 164 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cGm9a | 1 |
Entities:
Entity 1, cGm9a 30 residues - 3075.419 Da.
| 1 | SER | CYS | ASN | ASN | SER | CYS | GLN | SER | HIS | SER | |
| 2 | ASP | CYS | ALA | SER | HIS | CYS | ILE | CYS | THR | PHE | |
| 3 | ARG | GLY | CYS | GLY | ALA | VAL | ASN | GLY | LEU | PRO |
Samples:
sample_1: cGm9a0.05 – 0.2 mM; H2O 90%; D2O 10%
sample_conditions_1: pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker Avance 900 MHz