BMRB Entry 25119

Name: Chemical shift assignments for human cardiac troponin I, residues 1-73, bound to cardiac troponin C
Published: 2014-09-03

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25119

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shift assignments for human cardiac troponin I, residues 1-73, bound to cardiac troponin C

Deposition date:

2014-07-31

Original release date:

2014-09-03

Authors:

Hwang, Peter

Citation:

Citation: Hwang, Peter. "cTnI[1-73] bound to cTnC" Proc. Natl. Acad. Sci. U.S.A. ., .-..

Assembly members:

Assembly members:
cTnI[1-73], polymer, 80 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-PagP

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cTnI[1-73]: LADGSSDAAREPRPAPAPIR RRSSNYRAYATEPHAKKKSK ISASRKLQLKTLLLQIAKQE LEREAEERRGEKGRHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	434
13C chemical shifts	282
15N chemical shifts	67

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	cTnI[1-73]-cTnC	1

Entities:

Entity 1, cTnI[1-73]-cTnC 80 residues - Formula weight is not available

1	LEU	ALA	ASP	GLY	SER	SER	ASP	ALA	ALA	ARG
2	GLU	PRO	ARG	PRO	ALA	PRO	ALA	PRO	ILE	ARG
3	ARG	ARG	SER	SER	ASN	TYR	ARG	ALA	TYR	ALA
4	THR	GLU	PRO	HIS	ALA	LYS	LYS	LYS	SER	LYS
5	ILE	SER	ALA	SER	ARG	LYS	LEU	GLN	LEU	LYS
6	THR	LEU	LEU	LEU	GLN	ILE	ALA	LYS	GLN	GLU
7	LEU	GLU	ARG	GLU	ALA	GLU	GLU	ARG	ARG	GLY
8	GLU	LYS	GLY	ARG	HIS	HIS	HIS	HIS	HIS	HIS

Samples:

sample_1: cTnI[1-73], [U-100% 13C; U-100% 15N], 0.5 mM; cTnC 0.5 mM; H20 95%; D20 5%

sample_conditions_1: temperature: 313 K; pH: 6.2; pressure: 1 atm; ionic strength: 0.15 M

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz
Varian INOVA 800 MHz

BMRB	25118
DBJ	BAD97171
EMBL	CAA38102 CAA62301 CAG46782 CAG46809
GB	AAA16157 AAH96165 AAH96166 AAH96167 AAH99631
PRF	1616225A
REF	NP_000354 XP_003807165 XP_004061509 XP_009434690
SP	P19429
AlphaFold	P19429

1	LEU	ALA	ASP	GLY	SER	SER	ASP	ALA	ALA	ARG
2	GLU	PRO	ARG	PRO	ALA	PRO	ALA	PRO	ILE	ARG
3	ARG	ARG	SER	SER	ASN	TYR	ARG	ALA	TYR	ALA
4	THR	GLU	PRO	HIS	ALA	LYS	LYS	LYS	SER	LYS
5	ILE	SER	ALA	SER	ARG	LYS	LEU	GLN	LEU	LYS
6	THR	LEU	LEU	LEU	GLN	ILE	ALA	LYS	GLN	GLU
7	LEU	GLU	ARG	GLU	ALA	GLU	GLU	ARG	ARG	GLY
8	GLU	LYS	GLY	ARG	HIS	HIS	HIS	HIS	HIS	HIS

1	LEU	ALA	ASP	GLY	SER	SER	ASP	ALA	ALA	ARG
2	GLU	PRO	ARG	PRO	ALA	PRO	ALA	PRO	ILE	ARG
3	ARG	ARG	SER	SER	ASN	TYR	ARG	ALA	TYR	ALA
4	THR	GLU	PRO	HIS	ALA	LYS	LYS	LYS	SER	LYS
5	ILE	SER	ALA	SER	ARG	LYS	LEU	GLN	LEU	LYS
6	THR	LEU	LEU	LEU	GLN	ILE	ALA	LYS	GLN	GLU
7	LEU	GLU	ARG	GLU	ALA	GLU	GLU	ARG	ARG	GLY
8	GLU	LYS	GLY	ARG	HIS	HIS	HIS	HIS	HIS	HIS