BMRB Entry 25106

Title:
Structural dynamics of double-helical RNA having CAG motif
Deposition date:
2014-07-23
Original release date:
2015-01-26
Authors:
KUMAR, AMIT; TAWANI, ARPITA
Citation:

Citation: KUMAR, AMIT; TAWANI, ARPITA. "Structural Insight reveals dynamics in repeating r(CAG) transcript found in Huntington s disease (HD) and Spinocerebellar ataxias (SCAs)"  .

Assembly members:

Assembly members:
RNA_(5'-R(P*CP*CP*GP*CP*AP*GP*CP*GP*G)-3'), polymer, 9 residues, 2885.816 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Experimental source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA_(5'-R(P*CP*CP*GP*CP*AP*GP*CP*GP*G)-3'): CCGCAGCGG

Data sets:
Data typeCount
1H chemical shifts33

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA (5'-R(P*CP*CP*GP*CP*AP*GP*CP*GP*G)-3')_11
2RNA (5'-R(P*CP*CP*GP*CP*AP*GP*CP*GP*G)-3')_21

Entities:

Entity 1, RNA (5'-R(P*CP*CP*GP*CP*AP*GP*CP*GP*G)-3')_1 9 residues - 2885.816 Da.

1   CCGCAGCGG

Samples:

sample_1: RNA (5'-R(P*CP*CP*GP*CP*AP*GP*CP*GP*G)-3') 2.35 mM; H2O 90%; D2O 10%; Phosphate buffer 10 mM; NaCl 100 mM; EDTA 0.05 mM

sample_conditions_1: temperature: 308 K; pH: 7.2; pressure: 1 atm; ionic strength: 0.018 M

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

DISCOVER, Accelrys Software Inc. - structure solution

NMR spectrometers:

  • Bruker Avance 400 MHz
  • Bruker Avance 700 MHz