BMRB Entry 25105

Name: BAZ2B bromodomain
Published: 2022-05-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25105

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

BAZ2B bromodomain

Deposition date:

2014-07-23

Original release date:

2022-05-12

Authors:

Dias, David; Wienk, Hans; Ciulli, Alessio

Citation:

Citation: Ferguson, Fleur; Dias, David; Rodrigues, Joao; Wienk, Hans; Boelens, Rolf; Bonvin, Alexandre; Abell, Chris; Ciulli, Alessio. "Binding hotspots of BAZ2B bromodomain: Histone interaction revealed by solution NMR driven docking" Biochemistry 53, 6706-6716 (2014).
PubMed: 25266743

Assembly members:

Assembly members:
BAZ2B, polymer, 117 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pNIC28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BAZ2B: SMSVKKPKRDDSKDLALCSM ILTEMETHEDAWPFLLPVNL KLVPGYKKVIKKPMDFSTIR EKLSSGQYPNLETFALDVRL VFDNCETFNEDDSDIGRAGH NMRKYFEKKWTDTFKVS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	311
15N chemical shifts	108
1H chemical shifts	108

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	BAZ2B	1

Entities:

Entity 1, BAZ2B 117 residues - Formula weight is not available

1

SER

MET

SER

VAL

LYS

PRO

LYS

ARG

ASP

2

ASP

SER

LYS

ASP

LEU

ALA

LEU

CYS

SER

MET

3

ILE

LEU

THR

GLU

MET

GLU

THR

HIS

GLU

ASP

4

ALA

TRP

PRO

PHE

LEU

PRO

VAL

ASN

LEU

5

LYS

LEU

VAL

PRO

GLY

TYR

LYS

VAL

ILE

6

LYS

PRO

MET

ASP

PHE

SER

THR

ILE

ARG

7

GLU

LYS

LEU

SER

GLY

GLN

TYR

PRO

ASN

8

LEU

GLU

THR

PHE

ALA

LEU

ASP

VAL

ARG

LEU

9

VAL

PHE

ASP

ASN

CYS

GLU

THR

PHE

ASN

GLU

10

ASP

SER

ASP

ILE

GLY

ARG

ALA

GLY

HIS

11

ASN

MET

ARG

LYS

TYR

PHE

GLU

LYS

TRP

12

THR

ASP

THR

PHE

LYS

VAL

SER

Samples:

sample_1: BAZ2B, [U-95% 13C; U-95% 15N], 1 ± 0.1 mM; H20 90%; D2O 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.5; pressure: ambient atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks