BMRB Entry 25084

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Sp Cdc5-D3
Published: 2019-07-11

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25084

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Sp Cdc5-D3

Deposition date:

2014-07-10

Original release date:

2019-07-11

Authors:

Collier, Scott

Citation:

Citation: Collier, Scott; Voehler, Markus; Peng, Dungeng; Ohi, Ryomi; Gould, Kathleen; Reiter, Nicholas; Ohi, Melanie. "Structural and functional insights into the N-terminus of Schizosaccharomyces pombe Cdc5" Biochemistry 53, 6439-6451 (2014).
PubMed: 25263959

Assembly members:

Assembly members:
Cdc5-D3_(aa_155-214), polymer, 63 residues, 7471.6 Da.

Natural source:

Natural source: Common Name: E.coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15-b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cdc5-D3_(aa_155-214): GSHMDEDEKEMLSEARARLA NTQGKKAKRKDREKQLELTR RLSHLQKRRELKAAGINIKL FRR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	184
15N chemical shifts	60
1H chemical shifts	60

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Cdc5-D3 monomer	1

Entities:

Entity 1, Cdc5-D3 monomer 63 residues - 7471.6 Da.

1

GLY

SER

HIS

MET

ASP

GLU

ASP

GLU

LYS

GLU

2

MET

LEU

SER

GLU

ALA

ARG

ALA

ARG

LEU

ALA

3

ASN

THR

GLN

GLY

LYS

ALA

LYS

ARG

LYS

4

ASP

ARG

GLU

LYS

GLN

LEU

GLU

LEU

THR

ARG

5

ARG

LEU

SER

HIS

LEU

GLN

LYS

ARG

GLU

6

LEU

LYS

ALA

GLY

ILE

ASN

ILE

LYS

LEU

7

PHE

ARG

Samples:

Cdc5-D3_(aa_155-214)_sample: Cdc5-D3 (aa 155-214), [U-99% 13C; U-99% 15N], 370 uM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	Cdc5-D3_(aa_155-214)_sample	anisotropic	sample_conditions_1
3D HNCO	Cdc5-D3_(aa_155-214)_sample	anisotropic	sample_conditions_1
3D HNCA	Cdc5-D3_(aa_155-214)_sample	anisotropic	sample_conditions_1
3D HNCACB	Cdc5-D3_(aa_155-214)_sample	anisotropic	sample_conditions_1
3D HN(CO)CA	Cdc5-D3_(aa_155-214)_sample	anisotropic	sample_conditions_1
3D CBCA(CO)NH	Cdc5-D3_(aa_155-214)_sample	anisotropic	sample_conditions_1
3D HN(CA)CO	Cdc5-D3_(aa_155-214)_sample	anisotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

UNP	P39964
AlphaFold	P39964