BMRB Entry 25079

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25079
MolProbity Validation Chart

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Title:
Assignment of the transmembrane domain of the erythropoietin receptor
Deposition date:
2014-07-05
Original release date:
2014-12-08
Authors:
Li, Qingxin; Wong, Ying Lei; Huang, Qiwei; Kang, Congbao
Citation:

Citation: Li, Qingxin; Wong, Ying Lei; Huang, Qiwei; kang, congbao. "Structural insight into the transmembrane domain and the juxtamembrane region of the erythropoietin receptor in micelles"  Biophys. J. 107, 2325-2336 (2014).
PubMed: 25418301

Assembly members:

Assembly members:
human_EpoR, polymer, 55 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human_EpoR: MSEPVSLLTPSDLDPLILTL SLILVVILVLLTVLALLSHR RALKQKIWPHHHHHH

Data sets:
Data typeCount
13C chemical shifts90
1H chemical shifts170
15N chemical shifts42

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1human Epo1

Entities:

Entity 1, human Epo 55 residues - Formula weight is not available

1   METSERGLUPROVALSERLEULEUTHRPRO
2   SERASPLEUASPPROLEUILELEUTHRLEU
3   SERLEUILELEUVALVALILELEUVALLEU
4   LEUTHRVALLEUALALEULEUSERHISARG
5   ARGALALEULYSGLNLYSILETRPPROHIS
6   HISHISHISHISHIS

Samples:

15N: human EpoR, [U-100% 15N], 0.5 mM; sodium phosphate 20 mM; DPC 200 mM; H20 90%; D20 10%

13C-15N: human EpoR, [U-100% 13C; U-100% 15N], 0.2 – 0.8 mM; sodium phosphate 20 mM; DPC 200 mM; H20 90%; D20 10%

sample_3: human EpoR, [U-100% 13C; U-100% 15N; U-80% 2H], 0.5 mM; sodium phosphate 20 mM; DPC 200 mM; H20 90%; D20 10%

sample_conditions_1: temperature: 313 K; pH: 6.5; pressure: 1 atm; ionic strength: 20 mM

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
2D 1H-15N HSQC15Nisotropicsample_conditions_1
3D HNCA13C-15Nisotropicsample_conditions_1
3D HNCACB13C-15Nisotropicsample_conditions_1
3D HBHA(CO)NH13C-15Nisotropicsample_conditions_1
3D HCACO13C-15Nisotropicsample_conditions_1
3D HNCO13C-15Nisotropicsample_conditions_1
3D 1H-15N NOESY13C-15Nisotropicsample_conditions_1
3D 1H-15N NOESYsample_3isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ BAG37561 BAI45971
GB AAA52401 AAA52403 AAB23271 AAI12154 AFM52330
REF NP_000112 XP_001105833 XP_002828735 XP_003830541 XP_004060086
SP P19235
AlphaFold P19235

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