BMRB Entry 25036
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25036
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Ye, Kaiqin. "Solution structure of ubiquitin-like protein from Caldiarchaeum subterraneum" .
Assembly members:
entity, polymer, 88 residues, 8107.625 Da.
Natural source: Common Name: archaea Taxonomy ID: 311458 Superkingdom: Archaea Kingdom: not available Genus/species: Caldiarchaeum subterraneum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28 b (+)
Entity Sequences (FASTA):
entity: HMKIKIVPAVGGGSPLELEV
APNATVGAVRTKVCAMKKLP
PDTTRLTYKGRALKDTETLE
SLGVADGDKFVLITRTVGGC
GEPIRRAA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 244 |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 472 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CsUbl | 1 |
Entities:
Entity 1, CsUbl 88 residues - 8107.625 Da.
| 1 | HIS | MET | LYS | ILE | LYS | ILE | VAL | PRO | ALA | VAL | ||||
| 2 | GLY | GLY | GLY | SER | PRO | LEU | GLU | LEU | GLU | VAL | ||||
| 3 | ALA | PRO | ASN | ALA | THR | VAL | GLY | ALA | VAL | ARG | ||||
| 4 | THR | LYS | VAL | CYS | ALA | MET | LYS | LYS | LEU | PRO | ||||
| 5 | PRO | ASP | THR | THR | ARG | LEU | THR | TYR | LYS | GLY | ||||
| 6 | ARG | ALA | LEU | LYS | ASP | THR | GLU | THR | LEU | GLU | ||||
| 7 | SER | LEU | GLY | VAL | ALA | ASP | GLY | ASP | LYS | PHE | ||||
| 8 | VAL | LEU | ILE | THR | ARG | THR | VAL | GLY | GLY | CYS | ||||
| 9 | GLY | GLU | PRO | ILE | ARG | ARG | ALA | ALA |
Samples:
sample_1: entity, [U-100% 13C; U-100% 15N], 0.8 mM; sodium phosphate 1 mM; H2O 90%; D2O 10%
sample_2: entity, [U-100% 15N], 0.8 mM; sodium phosphate 1 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_2 | solution | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | solution | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | solution | sample_conditions_1 |
| 3D HNCACB | sample_1 | solution | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | solution | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | solution | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | solution | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker DMX 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks