BMRB Entry 25023

Name: 15N HSQC assignment of Drosophila ELF domain from FANCL
Published: 2016-07-01

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25023

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

15N HSQC assignment of Drosophila ELF domain from FANCL

Deposition date:

2014-06-16

Original release date:

2016-07-01

Authors:

Miles, Jennifer; Carroll, Ellis; Rowe, Michelle; Howard, Mark; Sihu, Ateesh; Frost, Mark; Williamson, Richard; Alpi, Arno; Walden, Helen

Citation:

Citation: Miles, Jennifer; Carroll, Ellis; Rowe, Michelle; Howard, Mark; Sidhu, Ateesh; Frost, Mark; Williamson, Richard; Alpi, Arno; Walden, Helen. "The Fanconi Anemia DNA Repair Pathway Is Regulated by an Interaction between Ubiquitin and the E2-like Fold Domain of FANCL" J. Biol. Chem. 290, 20995-21006 (2015).
PubMed: 26149689

Assembly members:

Assembly members:
ELF, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28a SUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ELF: MESNEDVERLLCQKYPGLAA ELQPSGACIIRGVLGSEDTW RRLKLYLPHHPALHGFQLYV QESLEYKLYTSANLKLQDDW LLEDFLDHLPKILPAQKAPT VPKE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	74
1H chemical shifts	81

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ELF	1

Entities:

Entity 1, ELF 104 residues - Formula weight is not available

1

MET

GLU

SER

ASN

GLU

ASP

VAL

GLU

ARG

LEU

2

LEU

CYS

GLN

LYS

TYR

PRO

GLY

LEU

ALA

3

GLU

LEU

GLN

PRO

SER

GLY

ALA

CYS

ILE

4

ARG

GLY

VAL

LEU

GLY

SER

GLU

ASP

THR

TRP

5

ARG

LEU

LYS

LEU

TYR

LEU

PRO

HIS

6

PRO

ALA

LEU

HIS

GLY

PHE

GLN

LEU

TYR

VAL

7

GLN

GLU

SER

LEU

GLU

TYR

LYS

LEU

TYR

THR

8

SER

ALA

ASN

LEU

LYS

LEU

GLN

ASP

TRP

9

LEU

GLU

ASP

PHE

LEU

ASP

HIS

LEU

PRO

10

LYS

ILE

LEU

PRO

ALA

GLN

LYS

ALA

PRO

THR

11

VAL

PRO

LYS

GLU

Samples:

sample_1: ELF, [U-99% 15N], 0.6 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Varian Unity 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks