BMRB Entry 21062
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR21062
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Citation: Yua, Shuo; Du, Tianpeng; Liu, Zhuguo; Wu, Qiaoling; Feng, Guixue; Dong, Mingxin; Zhou, Xiaowei; Jiang, Ling; Dai, Qiuyun. "Im10A, a short conopeptide isolated from Conus imperialis and possesses two highly concentrated disulfide bridges and analgesic activity." Peptides 81, 15-20 (2016).
PubMed: 27131596
Assembly members:
Im10A, polymer, 11 residues, 1239.472 Da.
Natural source: Common Name: Conus imperialis Taxonomy ID: 35631 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Conus imperialis
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Im10A: NTICCEGCMCY
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 56 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Im10A | 1 |
Entities:
Entity 1, Im10A 11 residues - 1239.472 Da.
| 1 | ASN | THR | ILE | CYS | CYS | GLU | GLY | CYS | MET | CYS | ||||
| 2 | TYR |
Samples:
sample_1: entity0.3 – 0.5 mM; sodium phosphate 20 mM; H2O 90%; D2O, [U-2H], 10%
sample_conditions_1: pH: 5.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CARA v1.5.5, Keller and Wuthrich - chemical shift assignment, data analysis
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
AMBER v11, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement
NMR spectrometers:
- Bruker Avance 800 MHz