BMRB Entry 21060

Title:
conotoxin Eb1.6
Deposition date:
2015-12-17
Original release date:
2022-03-01
Authors:
Jiang, Ling; Du, Tianpeng
Citation:

Citation: Liu, Zhuguo; Bartels, Peter; Sadeghi, Mahsa; Du, Tianpeng; Dai, Qing; Zhu, Cui; Yu, Shuo; Wang, Shuo; Dong, Mingxin; Sun, Ting; Guo, Jiabin; Peng, Shuangqing; Jiang, Ling; Adams, David; Dai, Qiuyun. "A novel alpha-conopeptide Eu1.6 inhibits N-type (Ca V 2.2) calcium channels and exhibits potent analgesic activity"  Sci. Rep. 8, 1004-1004 (2018).
PubMed: 29343689

Assembly members:

Assembly members:
entity, polymer, 16 residues, 1669.013 Da.

Natural source:

Natural source:   Common Name: Conus eburneus   Taxonomy ID: 101300   Superkingdom: Eukaryiota   Kingdom: Metazoa   Genus/species: Conus eburneus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GCCSNPACMLKNPNLC

Data sets:
Data typeCount
1H chemical shifts91

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Eb1.61

Entities:

Entity 1, Eb1.6 16 residues - 1669.013 Da.

1   GLYCYSCYSSERASNPROALACYSMETLEU
2   LYSASNPROASNLEUCYS

Samples:

sample_1: entity 0.5 mM; phosphate buffer 20 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: pH: 5.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz